球形棕囊藻与红树林细菌Flavobacterium sp.相互关系的研究

从深圳福田红树林中分离出一株细菌,对球形棕囊藻有溶藻活性,通过形态鉴定为假单胞菌属细菌。将球形棕囊藻、藻过滤液和提取的溶血毒素与细菌共培养,通过荧光显微计数及平板计数研究活细菌数和可培养细菌数,结果表明:当细菌直接与棕囊藻共培养时,细菌很快进入VBNC状态,细菌能够促进棕囊藻溶血毒素的积累。而将细菌置于透析袋中与棕囊藻一起共培养时,棕囊藻对细菌的VBNC状态影响不大,细菌对棕囊藻溶血毒素的积累无影响。对数期、稳定期棕囊藻培养过滤液和溶血毒素均可使细菌很快进入VBNC状态,溶血毒素可能在球形棕囊藻在被牧食及抑制其他竞争者中(化感作用)扮演着重要的角色。

Relationship of Phaeocystis globosa and mangrove bacteria Flavobacterium.sp

A bacteria was isolated from Futian mangrove in Shenzhen and was identified as Pseudomonas.spp.These bacteria had the algicidal activity on Phaeocystis globosa.When the bacteria was co-cultured with P.globosa,algae filtrate and hemolytic toxins,the number of live bacteria and cultivable bacteria were studied by method of fluorescence microscopy counting and plate counting.The results showed that when the bacteria were incubated in P.globosa,and co-cultured with P.globosa,the bacteria entered the VBNC state quickly.The bacteria could promote P.globosa the accumulation of hemolysin.When the bacteria cells were placed in dialysis bag and co-culture with P.globosa,the bacteria did not impact the hemolysin production also P.globosa,hardly effect on the VBNC state of the bacteria.Almost P.globosa filtrate of logarithmic phase and stationary phase and hemolytic toxins promoted the bacteria to entering the VBNC state.Hemolysin hemolysin may play an important role to prevent the grazing and inhibiting other competitors(allelopathy).