| 全期表达外源基因的重组杆状病毒 |
| |
| 引用本文： | 吴文言,龙綮新,钟俏芬,杨洁,王珣章.全期表达外源基因的重组杆状病毒[J].应用与环境生物学报,1999,5(5):521-524. |
| |
| 作者姓名： | 吴文言 龙綮新 钟俏芬 杨洁 王珣章 |
| |
| 作者单位： | 中山大学生物防治国家重点实验室,广州,510275 |
| |
| 摘 要： | 构建了含早期、晚期、极晚期启动子、全期表达ＨＢｓＡｇ 基因的重组杆状病毒ｖＴｎＮＰＶＰ３５ＨＢｓＯＣＣ＋ ．结果ＨＢｓＡｇ 表达时间由感染后１２ ｈ 提前到４ ｈ，而其表达量，特别是病毒感染早期的表达水平亦显著提高，其中２４ ｈ 和９６ ｈ 的表达量分别比对照提高１８０ ％ 和６０ ％ ． 转录起始位点分析结果显示，该重组毒株可在病毒感染的早、晚期形成多个转录本
|
| 关 键 词： | 杆状病毒 全期表达 转录起始位点 |
| 修稿时间： | 1999-01-27 |
WHOLE-PHASE EXPRESSION OF FOREIGN GENE IN A RECOMBINANT BACULOVIRUS |
| |
| WU Wenyan,LONG Qingxin,ZHONG Qiaofen,YAN Jie,WANG Xunzhang.WHOLE-PHASE EXPRESSION OF FOREIGN GENE IN A RECOMBINANT BACULOVIRUS[J].Chinese Journal of Applied and Environmental Biology,1999,5(5):521-524. |
| |
| Authors: | WU Wenyan LONG Qingxin ZHONG Qiaofen YAN Jie WANG Xunzhang |
| |
| Abstract: | A recombinant baculovirus vTnNPV P 35 HBs OCC was constructed, containing the HBsAg gene with the control of the early, late, and very late promoters. The expression products of HBsAg gene could be detected at 4 h postinfection. The expression level of HBsAg was increased by 180% and 60% compared with the control at 24 h and 96 h, respectively. The primer extension analysis was carried out and the initiate sites of promoter cassette were determined. |
| |
| Keywords: | baculovirus whole phase expression initiate sites |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
