Waste recombinant DNA: Effectiveness of thermo-treatment to manage potential gene pollution |
| |
| Authors: | Xiaohua Fu Mengnan Li Guanghong Zheng Yiquan Le Lei Wang |
| |
| Institution: | 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China;2. China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing 100081, China;3. R&D, China Resources Sanjiu Medical&Pharmaceutical Co., Ltd, Shenzhen 518110, China;4. The General Hospital of the People''s Liberation Army (Hospital 301), Beijing 100853, China;1. MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China;2. Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea |
| |
| Abstract: | Heating at 100 °C for 5–10 min is a common method for treating wastewater containing recombinant DNA in many bio-laboratories in China. In this experiment, plasmid pET-28b was used to investigate decay efficiency of waste recombinant DNA during thermo-treatment. The results showed that the decay half-life of the plasmid was 2.7–4.0 min during the thermo-treatment, and even heating for 30 min the plasmids still retained some transforming activity. Low pH promoted the decay of recombinant DNA, but NaCl, bovine serum albumin and EDTA, which existed in the most wastewater from bio-laboratories, protected DNA from degradation. Thus, the decay half-life of plasmid DNA may be longer than 2.7–4.0 min practically. These results suggest that the effectiveness of heating at 100 °C for treating waste recombinant DNA is low and a gene pollution risk remains when those thermo-treated recombinant DNAs are discharged into the environment. Therefore other simple and effective methods should be developed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
