DEHP暴露对小鼠神经行为学及脑脂质过氧化物的影响

为研究增塑剂邻苯二甲酸二乙基己酯(Di-2-ethylhexyl phthalate,DEHP)对脑组织造成氧化损伤以及对小鼠神经行为(主要包括学习、记忆能力与情绪)的影响.将24只雄性昆明小鼠随机分成4组,每组6只,分别为对照组(生理盐水),低浓度染毒组(5mg·kg-1DEHP,L组),中浓度染毒组(50mg·kg-1DEHP,M组),高浓度染毒组(500mg·kg-1DEHP,H组).利用Y型电迷宫检验小鼠的学习记忆能力变化,利用悬尾实验和强迫游泳实验检测小鼠抑郁行为的变化.然后取出脑组织,检验活性氧簇(ROS)及丙二醛(MDA)含量.数据显示,染毒组小鼠的学习记忆及行为学控制能力明显低于对照组(p<0.05),并且随DEHP浓度的增大小鼠的学习记忆及行为学控制能力降低.DEHP低、中、高剂量染毒组与对照组氧化应激反应均具有显著性(p<0.05),且呈剂量效应关系.因此,DEHP可导致小鼠学习记忆能力下降、抑郁情绪增加,其分子机制可能涉及DEHP对脑组织造成的氧化损伤.

Effect of di-(2-ethylhexyl)-phthalate exposure on neurobehavioral and lipid peroxidation of mice

Abstract: To study the effect of DEHP on neurobehavioral (the ability of learning, memory and emotion) of Kunming mice and the oxidative damage caused by DEHP in their brain. Dividing twenty-four male kunming mice into 4 groups (n=6) randomly, including control group (Saline), low density DEHP treatment group (5 mg·kg^-1 DEHP), medium density DEHP treatment group (50 mg·kg^-1 DEHP) and high density DEHP treatment group (500mg/kg DEHP). To begin with, test the ability of learning and memory by Y-electric maze. Then, use the forced swimming and tail suspension tests to learn the depression of mice. Finally, the ROS level, and MDA contents of brain tissues were measured. According to the data, the ability of learning and memory of the three treatment groups are apparently lower than the control group, while their depression is extreme worse than control group (p<0.05). What is more, comparing with the control group, the oxidative stress reactions of the three treatment groups are increasing significantly. The results suggest that DEHP does cause the oxidative damage in the mice's brains, which then results the abnormality of nervous behaviors.