| 一株耐盐苯酚降解菌的分离、鉴定及耐盐机制研究 |
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| 引用本文： | 沈娥,王平,周豪,孔春雷,马桥,曲媛媛,周集体. 一株耐盐苯酚降解菌的分离、鉴定及耐盐机制研究[J]. 环境科学学报, 2013, 33(2): 377-382 |
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| 作者姓名： | 沈娥 王平 周豪 孔春雷 马桥 曲媛媛 周集体 |
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| 作者单位： | 1. 大连理工大学环境学院,工业生态与环境工程教育部重点实验室,大连116024
2. 大连百奥泰科技有限公司,大连,116030 |
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| 基金项目： | 国家自然科学基金(No.21176040,51078054) |
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| 摘 要： | 从污泥样品中分离得到1株高效的耐盐苯酚降解菌,该菌株能够在温度20～30 ℃、pH5.0～9.0和盐度1％ ～ 10％范围内以苯酚为唯一碳源进行生长.通过形态、生理生化特性分析及16S rDNA序列分析,鉴定该菌株为Rhodococcus sp.,命名为W2.在温度30℃、pH =7.0、NaCl50g·L-1的条件下,菌株可耐受苯酚的最大浓度为700 mg·L-1.粗酶中检测到的邻苯二酚l,2-双加氧酶活性表明,菌株W2通过邻位开环断裂途径代谢苯酚.底物广谱性考察结果表明,菌株W2能够在高盐条件下利用苯甲酸、水杨酸等多种芳香化合物作为碳源生长.对耐盐机理进行的初步分析表明,细胞内甜菜碱和四氢嘧啶的含量随着盐浓度的增加而增大,说明四氢嘧啶和甜菜碱的积累是菌株W2抵抗高盐度的重要机制.
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| 关 键 词： | 生物降解 苯酚 红球菌属 耐盐机制 |
| 收稿时间： | 2012-04-09 |
| 修稿时间： | 2012-05-04 |
Isolation, identification and the salt-tolerant mechanism of a salt-tolerant strain for phenol degradation |
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| SHEN E,WANG Ping,ZHOU Hao,KONG Chunlei,MA Qiao,QU Yuanyuan and ZHOU Jiti. Isolation, identification and the salt-tolerant mechanism of a salt-tolerant strain for phenol degradation[J]. Acta Scientiae Circumstantiae, 2013, 33(2): 377-382 |
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| Authors: | SHEN E WANG Ping ZHOU Hao KONG Chunlei MA Qiao QU Yuanyuan ZHOU Jiti |
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| Affiliation: | Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024;Dalian BITeomics Inc., Dalian 116030;Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024;Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024;Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024;Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024;Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024 |
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| Abstract: | |
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| Keywords: | biodegradation phenol Rhodococcus sp. salt-tolerant mechanism |
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