基于DGAOs富集的内碳源短程硝化反硝化工艺特性

采用序批式生物反应器（SBR），以厌氧-好氧-缺氧的运行方式，研究了低C/N比下内碳源驱动的短程硝化反硝化工艺运行性能.结果表明，反应器内可同时富集反硝化聚糖菌（DGAOs）和氨氧化细菌（AOB）.DGAOs可以利用聚-β-羟基脂肪酸酯（PHA）为内碳源进行反硝化，且利用的PHA中PHB （聚-β-羟基丁酸酯）占主要部分.稳定运行后，第39d厌氧末期污泥胞内存储物质在荧光显微镜下清晰可见，内碳源存储的PHA在缺氧阶段净消耗量为2.34mmol C/L，较文献报道值高29%.经过55d的驯化后，SBR系统达到了较为稳定的脱氮效果，平均氨氮去除率为（93.13%±4.91%），内碳源反硝化效率为（49.62%±8.97%）.驯化后的污泥淘汰了反硝化聚磷菌（DPAOs）和亚硝酸盐氧化菌（NOB），富集了DGAOs和AOB，其丰度从接种时的0.13%和0.20%分别上升到7.13%和1.11%，实现了低C/N下内碳源驱动短程硝化反硝化.

Short-cut nitrification and denitrification process characteristics of internal carbon source based on DGAOs enrichment

The operation performance of short-cut nitrification and denitrification process driven by internal carbon source at low C/N ratio was studied using Sequencing Batch Reactor (SBR) and anaerobic-aerobic-anoxia operation mode. The results showed that denitrifying golyphosphate accumulating Organisms (DGAOs) and ammonia-oxidizing Bacteria (AOB) can be enriched simultaneously in the reactor. DGAOs can use poly-β-hydroxyalkanoate (PHA) as internal carbon source for denitrification, and PHB (poly-β-hydroxy butyrate) was the main part of PHA. After stable operation, intracellular substances stored in sludge at the end of anaerobic phase could be clearly seen under fluorescence microscope at the 39th day, and 2.34mmol C/L in PHA stored by internal carbon source was consumed, which was 29% higher than the value reported in literature. After 55 days of domestication, the SBR system achieved a relatively stable denitrification effect. The average ammonia nitrogen removal rate was 93.13%±4.91%, and the denitrification efficiency of internal carbon source was 49.62%±8.97%. After domestication, denitrifying phosphate accumulating organisms(DPAOs) and nitrite oxidizing bacteria(AOB) were eliminated, denitrifying glycan bacteria and ammonia-oxidizing bacteria were enriched, and their abundance increased from 0.13% and 0.20% at inoculation to 7.13% and 1.11%, respectively, realizing the short-cut nitrification and denitrification driven by internal carbon source at low C/N.