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As-resistance in laboratory-reared F1, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil |
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| Authors: | CJ Langdon AJ Morgan JM Charnock KT Semple CN Lowe |
| Institution: | a C/O The Open University in the North, Baltic Buiness Quarter, Abbots Hill, Gateshead NE8 3DF, UK b Cardiff School of Biosciences, Cardiff University, P.O. Box 913, Cardiff CF11 3TL, Wales, UK c STFC Daresbury Laboratory, Warrington, Cheshire WA4 4AD, UK d School of Earth, Atmospheric and Environmental Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, UK e Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK f School of Built and Natural Environment, University of Central Lancashire, Preston PR1 2HE, UK |
| Abstract: | Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg−1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC25 sodium arsenate (700 mg kg−1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC25 concentrations of sodium arsenate (50 mg kg−1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. |
| Keywords: | Earthworms Arsenic Exposure history Resistance Ligand-binding |
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