一种简便高效的土壤石油烃提取方法的建立

通过开展单因子实验与正交实验,考察不同参数下超声萃取法对土壤中总石油烃(TPH)的提取效率,并对比了超声萃取法、超声-索氏萃取法及传统索氏萃取法对不同原油污染土壤的提取效果。结果表明:超声萃取法的最优工作参数为20 m L二氯甲烷,超声10 min,4000 r/min离心10 min,萃取4次。3种提取方法中,超声萃取法对石油烃平均回收率最高,为100.95%,而传统的索氏萃取法最低,仅为94.54%。可见,超声萃取法操作简单,效率高,可广泛用于土壤中总石油烃含量的提取测定。

SIMPLE AND EFFICIENT EXTRACTION METHOD OF PETROLEUM HYDROCARBON IN SOIL

This study aimed to investigate the extracting efficiency of ultrasonic extraction of total petroleum hydrocarbon in soil with different parameters,by orthogonal test and single-factor tests.Besides that,the extraction rates of total petroleum hydrocarbon in soil by ultrasound extraction,ultrasound-soxhlet extraction and soxhlet extraction were compared,respectively.The results indicated that the optimization condition of ultrasound extraction was as follows:20 mL of methylene chloride as the extractant,ultrasonic treatment for 10 min,and centrifuged at 4 000 r/min for 10 min,repeated for 4 times.Different extracting method had significant influence on extraction rate of petroleum hydrocarbon,and the optimum extraction process was ultrasonic-centrifuged method,whose extraction rate was 100.95％.While the rate of soxhlet extraction was the lowest,only 94.54％.Thus,the method of ultrasonic extraction was simple and efficient,which could be widely used in measuring the content of total petroleum hydrocarbon in soil.