一株焦化废水降酚菌的质粒初步研究

从处理焦化废水的活性污泥池中，分离得到了一株具有降酚能力的细菌， Ｊ Ａ． Ｊ Ａ 可以在含酚的 Ｈ Ｐ 复杂培养基中生长，也可以在以酚为唯一碳源的 Ａ Ｂ Ｐ 培养基中生长，且具有产黑色素的特点．用碱裂解提取质粒的方法发现它含有一个质粒，定名为ｐ Ｘ Ｈ１ ，并测定了这个质粒的大小约为２ ．６ ｋｂ ．用限制性内切酶 Ｂｇｌ Ｉ和 Ｒｓａ Ｉ进行双酶切，作出了初步的物理图谱．ｐ Ｘ Ｈ１ 经过２０ 次没有酚作为选择压传代培养后仍然能１００ ％ 保留，同时， Ｊ Ａ菌仍具有降酚能力，表明其具有很高的遗传稳定性

CHARACTERIZATION OF pXH1, AN INDIGENOUS PLASMID OF PHENOL DEGRADING STRAIN JA ISOLATED FROM THE WASTE WATER OF A COKE PLANT

A bacterial strain JA was isolated and it which could grow on phenol as a sole carbon source in waste water from a coke plant. An indigenous plasmid was discovered by using alkali lysis method and named pXH1 with a size about 2.6 kb. A preliminary physical map was made by double cutting with restriction enzymes Bgl I and Rsa I. After transfer of culture without phenol as selective pressure for 20 times, 100% of pXH1 was still maintained in the bacterium and JA still had the capacity of degrading phenol. These results showed that pXH1 had high genetic stability. The relationship between phenol degration and the plasmid needs further study. This plasmid may be useful in construction of new vectors for genetic engineering of phenol degrading bacteria.