An evaluation of the applicability of microarrays for monitoring toxic algae in Irish coastal waters |
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Authors: | Gary R McCoy Nicolas Touzet Gerard TA Fleming Robin Raine |
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Affiliation: | 3. The Ryan Institute, National University of Ireland, Galway, University Road, Galway, Ireland 1. School of Science, Department of Environmental Science, Institute of Technology, Sligo, Ireland 2. The Ryan Institute, Discipline of Microbiology, School of Natural Science, National University of Ireland, Galway, Ireland
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Abstract: | The applicability of microarrays to monitor harmful algae across a broad range of ecological niches and toxic species responsible for harmful algal events has been one of the key tasks in the EU Seventh Framework Programme (FP7)-funded Microarrays for the Detection of Toxic Algae project. The technique has a strong potential for improving speed and accuracy of the identification of harmful algae and their toxins to assist monitoring programmes. Water samples were collected from a number of coastal sites around Ireland, including several that are used in the Irish National Phytoplankton and Biotoxin Monitoring Programme. Ribosomal RNA was extracted from filtered field samples, labelled with a fluorescent dye, and hybridised to probes spotted in a microarray format on a glass slide. The fluorescent signal intensity of the hybridisation to >120 probes on the chip was analysed and compared with actual field counts. There was a general agreement between cell counts and microarray signal. Results are presented for field samples taken from a range of stations along the Irish coastline known for harmful algal events during the first field trial (July 2009–April 2010). |
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