| 苯酚微生物降解的正交实验研究 |
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| 引用本文： | 王闻烨,东天,马溪平,李鲜珠,李万龙,徐成斌. 苯酚微生物降解的正交实验研究[J]. 环境科学与管理, 2014, 39(8): 57-60 |
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| 作者姓名： | 王闻烨 东天 马溪平 李鲜珠 李万龙 徐成斌 |
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| 作者单位： | 辽宁大学环境学院,辽宁沈阳,110036 |
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| 基金项目： | 国家十二五科技重大专项子课题，辽宁大学本科教学改革研究项目，辽宁大学环境学院重点学科资助项目 |
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| 摘 要： | 利用以苯酚为碳源的驯化液,对某焦化厂曝气池活性污泥进行驯化,经过分离、筛选,挑选出4株高效的苯酚降解菌,编号为h32a2、b31B、h31A和b41a,并通过菌落形态特征、简单染色及生理生化反应初步鉴定为假单胞菌属(Pseudomonas sp.),依据正交实验确定了优势菌群降解苯酚的最佳条件为温度32℃,pH值7.5,菌培养时间16 h,接种量1%。
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| 关 键 词： | 苯酚 菌株 微生物降解 正交实验 |
Study on Biodegrading Phenol by Orthogonal Experiment |
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| Wang Wenye,Dong Tian,Ma Xiping,Li Xianzhu,Li Wanlong,Xu Chengbin. Study on Biodegrading Phenol by Orthogonal Experiment[J]. Environmental Science and Management, 2014, 39(8): 57-60 |
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| Authors: | Wang Wenye Dong Tian Ma Xiping Li Xianzhu Li Wanlong Xu Chengbin |
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| Affiliation: | (School of Environment Science, Liaoning University, Shenyang 110036, China) |
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| Abstract: | Four phenol degrading bacteria h32a2, b31B, h31A and b41a were isolated and screened from the activated sludge of aeration tank in coking plant, which were able to grow on phenol as carbon sources. The strains were identified as Pseudomonas sp. through colony morphology, physiological and biochemical responses and simple staining. Based on the orthogonal experiment, the best condition of each strain for degrading phenol were determined. The optimal degradation conditions were as follows： temperature 32℃, pH value 7.5, bacteria culture time 16h, inoculum 1%. |
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| Keywords: | phenol strain biodegradation orthogonal experiment |
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