利用EIA生物测试法快速定量筛选环境样品中的二噁污染物

将采集的环境样品分作 3份 ,1份用于高分辨色质联用多离子检测测定二噁的毒性当量浓度 TEQ,另 2份分别用 7-乙氧基 -异吩噁唑酮 -脱乙基酶 ( EROD)活力诱导法和酶免疫法 ( Enzyme Immuno Assay,EIA)进行生物测试 .结果表明 EIA和EROD这 2种生物试验方法均具有较好的准确性和很宽的线性范围 .比较 Micro- EROD分析结果与化学分析结果以及 EIA分析结果与化学分析结果 ,发现 Micro- EROD生物试验所测得的 TEQ值均高于化学分析法的 TEQ值 .其比值 1.83～ 3.06,平均比值 2.54± 0.59.而 EIA所测得的 TEQ值与化学结果的比率 0.5～1.4,平均比率为 0.83±0.40.显然由 EIA所测得的 TEQ的平均值比由 Micro- EROD所测得的结果更接近化学分析结果 .因此 EIA法更适合于对环境样品中二噁类化合物进行快速定量筛选 .

A New Enzyme Immunoassay for TEQ Screening of Dioxin/Furan in Environmental Samples

The collected environmental sample was divided into three parts,one of which was for TEQ determination by HRGC/HRMSMID,the other two parts was measured by Enzyme Immunoassay (EIA) and EROD bioassay respectively.The TEQs from EIA and EROD bioassay have a good relationship with those from chemical analysis,suggesting that both EIA and EROD bioassay are reliable for TEQ measurement.When the bioassay TEQs,which derived from EIA and EROD,were compared with results from chemical analysis respectively,the ERODTEQs(TEQ_EROD) are higher than the PCDD/FTEQs from chemical analysis (TEQ_PCDD/F).The ratios between TEQ_EROD and TEQ_PCDD/F lay between 1.83 to 3.06;the average ratio of TEQ_EROD/TEQ_PCDD/F is 1.83±059.However,for the EIA assay,the TEQ_EIA is around the value from chemical analysis.The ratio between TEQ_EIA and TEQ_PCDD/F is 050 to 1.40;the average ratio of TEQ_BIA/TEQ_PCDD/F is 088±040 It is obvious that the discrepancy between TEQ_EIA and the TEQ_PCDD/F is smaller than value between TEQ_EROD and TEQ_PCDD/F.This result reflects that the EIA assay should be more useful for a rapid and sensitive screening of environmental samples in many situations.