低氧条件下好氧型微生物降解煤吸附甲烷实验

为了研究好氧型微生物对低氧气浓度环境条件下煤吸附甲烷的降解效能，培养、分离、初步鉴定了高效降解甲烷的好氧型甲烷氧化菌。并在高压容量法瓦斯吸附-解吸装置的基础上，自主开发了低氧环境下甲烷降解实验分析系统，研究了氧气浓度为0%、5%和15%三种条件下甲烷氧化菌的降解效能，通过对实验前后甲烷减少量，二氧化碳增加量，氧气减少量进行分析。结果表明:在氧气浓度（0%~15%）范围内，随着氧气浓度升高及降解时间的持续，甲烷的减少量可达130.5 cm3，二氧化碳的增加量最高可达25.7 cm3，同时最多消耗69.0 cm3氧气；在无氧条件下，好氧型甲烷氧化菌的生理活性受到了一定的限制，但最高仍然可以降解11.9 cm3的甲烷，生成二氧化碳3.5 cm3。

Experiment on degradation of methane adsorption in coal by aerobic microorganism under low oxygen condition

In order to study the degradation efficiency of aerobic microorganism on methane adsorption in coal under low oxygen environment condition, the aerobic methane oxidizing bacteria for degradation of methane with high efficiency was cultivated, isolated and preliminary identified. An experimental analysis system on methane degradation under low oxygen environment was self-developed based on the methane adsorption and desorption device using high-pressure volumetric method, and the degradation efficiencies of methane oxidizing bacteria under the conditions of 0%, 5% and 15% oxygen concentration were studied. By comparing the decrement of methane, increment of carbon dioxide and decrement of oxygen before and after experiment, it showed that in the range of oxygen concentration (0~15%), with the increase of oxygen concentration and the duration of degradation time, the decrement of methane could reach 130.5 cm3, the maximum increment of carbon dioxide could reach 25.7 cm3, while 69 cm3 oxygen could be consumed at most. Under the condition of anaerobic environment, the physiological activity of aerobic methane oxidizing bacteria was subject to certain restrictions, but the maximum degradation amount of methane could still reach 11.9 cm3, and 3.5 cm3 carbon dioxide was produced.