| 一株降解原油链霉菌的分离鉴定与降解特性研究 |
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| 引用本文： | 司美茹,江翠翠,刘飞,赵云峰.一株降解原油链霉菌的分离鉴定与降解特性研究[J].生态与农村环境学报,2010,26(5). |
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| 作者姓名： | 司美茹 江翠翠 刘飞 赵云峰 |
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| 作者单位： | 曲阜师范大学生命科学学院,山东,曲阜,273165 |
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| 基金项目： | 山东省自然科学基金，曲阜师范大学本科科研训练项目 |
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| 摘 要： | 从山东东营胜利油田附近被石油污染的土壤中分离得到1株高效原油降解菌Z1a-B,依据形态和培养特征,初步鉴定Z1a-B菌株为链霉菌属白孢类群。试验结果表明,该菌株具有较强的溶血和排油活性,说明其产生生物表面活性剂的能力较强。通过气相色谱-质谱联用仪(GC-MS)分析,菌株能基本降解C12~C34的正构烷烃,对烷基苯、菲、甲基菲、萘也具有较强的降解能力。固体培养基配比以麸皮2 g、鸡粪40 g、草炭52.5 g、生石灰1.5g,或麸皮2 g、大米20 g、黄豆粉28 g、生石灰1.5 g较佳,在这2种配比培养基中链霉菌生长快,长势好,产孢子量多。经室内培养试验发现,当土壤中w(原油)=10%时,经Z1a-B菌株处理(35℃,pH 6.5)50 d,土壤原油降解率为67.5%。
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| 关 键 词： | 石油 降解 生物表面活性剂 放线菌 |
Isolation and Identification of a Crude-Oil-Degrading Strain of Streptomyces spp. and Its Oil-Degrading Characteristics |
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| SI Mei-ru,JIANG Cui-cui,LIU Fei,ZHAO Yun-feng.Isolation and Identification of a Crude-Oil-Degrading Strain of Streptomyces spp. and Its Oil-Degrading Characteristics[J].Journal of Ecology and Rural Environment,2010,26(5). |
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| Authors: | SI Mei-ru JIANG Cui-cui LIU Fei ZHAO Yun-feng |
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| Institution: | SI Mei-ru,JIANG Cui-cui,LIU Fei,ZHAO Yun-feng(College of Life Science,Qufu Normal University,Qufu 273165,China) |
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| Abstract: | A high-efficiency petroleum-degrading strain,Z1a-B,was isolated from the petroleum-polluted soil in Shengli Oil Field at Dongying,Shandong.By analyzing the morphology and cultural characteristics,Strain Z1a-B was identified as Albosporus in Streptomyces.Strain Z1a-B was tested to be quite strong in hemolyzing and petro-degrading activity,which implied that it was quite high in ability to produce biosurfactant.GC-MS analysis showed that the strain could almost completely degrade C12-C43 n-alkyl benzene and w... |
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| Keywords: | petroleum degradation biosurfactant screening Streptomyces spp |
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