| 农杆菌介导的抗除草剂基因转入两系杂交稻恢复系的研究 |
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| 引用本文： | 胡国成,肖晗,于永红,朱正歌,斯华敏,付亚萍,孙宗修. 农杆菌介导的抗除草剂基因转入两系杂交稻恢复系的研究[J]. 应用与环境生物学报, 2000, 6(6): 511-515 |
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| 作者姓名： | 胡国成 肖晗 于永红 朱正歌 斯华敏 付亚萍 孙宗修 |
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| 作者单位： | 中国水稻研究所农业部水稻生物学重点实验室杭州 310006 |
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| 基金项目： | 国家863计划重大项目“两系杂交水稻研究”资助(No:Z-16-02-04) |
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| 摘 要： | 利用农杆菌水稻高效转化系统，成功地将外源ＢＡＲ基因转入实用性两系要交稻恢复系Ｒ１８７和秀水０４，经过除草剂Ｂａｓｔａ田间涂布试验，其阳性率达到９０％以上。对转基因植株同步进行的ＰＣＲ特异扩增和总ＤＮＡ Ｓｏｕｔｈｅｒｎ检测结果也证实抗Ｂａｓｔａ的植株中含有ＢＡＲ基因。讨论了应用转ＢＡＲ基因的方法提高两系杂交水稻制种纯度的条件。图２表１参３
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| 关 键 词： | 两系杂交稻 恢复系 农杆菌转化 抗除草剂基因 |
| 修稿时间： | 2000-04-26 |
AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE RESTORER LINES OF TWO-LINE HYBRID RICE WITH BAR GENE |
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| HU Guocheng,XIAO Han,Yu Yonghong,ZHU Zhengge,SI Huamin,FU Yaping,SUN Zongxiu. AGROBACTERIUM-MEDIATED TRANSFORMATION OF THE RESTORER LINES OF TWO-LINE HYBRID RICE WITH BAR GENE[J]. Chinese Journal of Applied and Environmental Biology, 2000, 6(6): 511-515 |
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| Authors: | HU Guocheng XIAO Han Yu Yonghong ZHU Zhengge SI Huamin FU Yaping SUN Zongxiu |
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| Abstract: | An efficient Agrobacterium -mediated transformation protocol for rice was established in our laboratory. BAR gene was transferred into R187 and Xiushui 04. Two restorer lines of two line hybrid rice, using this protocol and more than 120 transgenic plants were regenerated. The exogenous BAR gene in those transgenic plants that expressed resistance to herbicide Basta was confirmed with PCR and Southern blot analysis. The limitation of this method for purifying two line hybrid rice seeds was also discussed. Fig 2, Tab 1 , Ref 13 |
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| Keywords: | Agrobacterium-mediated transformation BAR gene two-line hybrid rice restorer line |
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