| 黄土高原土壤中细菌群落结构多样性的PCR-DGGE分析 |
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| 引用本文： | 潘雪莲,黄晟,方昊,徐军,郭晓峰,陈旸,崔益斌. 黄土高原土壤中细菌群落结构多样性的PCR-DGGE分析[J]. 生态与农村环境学报, 2009, 25(3) |
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| 作者姓名： | 潘雪莲 黄晟 方昊 徐军 郭晓峰 陈旸 崔益斌 |
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| 作者单位： | 污染控制与资源化研究国家重点实验室/南京大学环境学院,江苏,南京,210093;南京大学地球科学与工程学院,江苏,南京,210093 |
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| 基金项目： | 国家自然科学基金重点项目 |
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| 摘 要： | 利用细菌通用引物,对从黄土高原5个地区土壤样品以及西峰剖面26个土壤样品中提取的总DNA进行聚合酶链式反应(PCR)扩增,采用变性梯度凝胶电泳(DGGE)技术对PCR产物进行分析,以揭示黄土高原土壤中细菌群落结构的多样性.针对黄土高原土壤微生物特点进行DGGE试验条件优化,获得最佳变性梯度为40%～70%,最佳电泳时间为17 h(100 V).对不同深度土壤细菌DGGE图谱的聚类分析表明,不同深度土壤细菌呈现2种不同的群落结构,推测其成因可能与季风性气候引起的温湿环境变化及冰期有关.在夏季风加强而冬季风减弱的时期,气候温暖潮湿,风化成壤作用强烈,在DGGE图谱中表现为土壤样品条带多而密;反之,风化成壤作用较弱,在DGGE图谱中表现为条带少而疏.对洛川、西峰等5个地区土壤细菌Shannon-Weiner指数的测定结果表明,Shannon-Weiner指数受条带亮度及迁移率的影响.
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| 关 键 词： | 黄土高原 微生物多样性 群落结构 PCR-DGGE |
Diversity of Bacterial Community Structure in Soils of Loess Plateau |
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| PAN Xue-lian,HUANG Sheng,FANG Hao,XU Jun,GUO Xiao-feng,CHEN Yang,CUI Yi-bin. Diversity of Bacterial Community Structure in Soils of Loess Plateau[J]. Journal of Ecology and Rural Environment, 2009, 25(3) |
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| Authors: | PAN Xue-lian HUANG Sheng FANG Hao XU Jun GUO Xiao-feng CHEN Yang CUI Yi-bin |
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| Affiliation: | 1.State Key Laboratory of Pollution Control and Resource Reuse/School of the Environment;Nanjing University;Nanjing 210093;China;2.School of Earth Sciences and Engineering;China |
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| Abstract: | Soil samples were collected from five regions and 26 soil samples from the Xifeng Profile of the Loess Plateau.Total DNA was extracted from those samples with the aid of bacterial universal primer and amplified by means of PCR and its product analyzed with the DGGE technique to explore diversity of bacterial communities.Oriented towards the features of the microbes in the soil of the plateau,conditions of the DGGE experiment were optimized with an optimal denaturant gradient ranging between 40% and 70%,and ... |
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| Keywords: | PCR-DGGE Loess Plateau bacterial diversity community structure PCR-DGGE |
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