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Ecotoxicological assessment of TiO2 byproducts on the earthworm Eisenia fetida |
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| Authors: | Bigorgne Emilie Foucaud Laurent Lapied Emmanuel Labille Jérôme Botta Céline Sirguey Catherine Falla Jaïro Rose Jérôme Joner Erik J Rodius François Nahmani Johanne |
| Institution: | a Laboratoire Interactions Ecotoxicité, Biodiversité, Ecosystèmes, Université Paul Verlaine - Metz, CNRS UMR 7146, Rue du Général Delestraint, 57070 Metz, France b Bioforsk, Soil and Environment, Fredrik A. Dahls vei 20, N-1432 Aas, Norway c CEREGE UMR 6635 CNRS/Aix-Marseille Université, Europôle de l’Arbois, 13545 Aix-en-Provence, France d International Consortium for the Environmental Implications of Nanotechnology iCEINT, Europole de l’Arbois, 13545 Aix en Provence, France e Nancy Université, INPL/INRA, UMR 1120, Laboratoire Sols et Environnement, BP 172-2, Avenue de la forêt de Haye, F-54505 Vandœuvre-lès-Nancy Cedex, France |
| Abstract: | The increasing production of nanomaterials will in turn increase the release of nanosized byproducts to the environment. The aim of this study was to evaluate the behaviour, uptake and ecotoxicity of TiO2 byproducts in the earthworm Eisenia fetida. Worms were exposed to suspensions containing 0.1, 1 and 10 mg/L of byproducts for 24 h. Size of TiO2 byproducts showed aggregation of particles up to 700 μm with laser diffraction. Only worms exposed at 10 mg/L showed bioaccumulation of titanium (ICP-AES), increasing expression of metallothionein and superoxide dismutase mRNA (Real-time PCR) and induction of apoptotic activity (Apostain and TUNEL). TiO2 byproducts did not induce cytotoxicity on cœlomocytes, but a significant decrease of phagocytosis was observed starting from 0.1 mg/L. In conclusion, bioaccumulation of byproducts and their production of reactive oxygen species could be responsible for the alteration of the antioxidant system in worms. |
| Keywords: | Nanocomposite TiO2 byproducts Earthworm Biomarker |
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