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Ozone affects pollen viability and NAD(P)H oxidase release from Ambrosia artemisiifolia pollen |
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| Authors: | Pasqualini Stefania Tedeschini Emma Frenguelli Giuseppe Wopfner Nicole Ferreira Fatima D'Amato Gennaro Ederli Luisa |
| Institution: | a Department of Applied Biology, University of Perugia, Perugia, Italy b Department of Molecular Biology, CD Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria c Division of Respiratory and Allergic Diseases, ’A. Cardarelli’ High Speciality Hospital, Naples, Italy |
| Abstract: | Air pollution is frequently proposed as a cause of the increased incidence of allergy in industrialised countries. We investigated the impact of ozone (O3) on reactive oxygen species (ROS) and allergen content of ragweed pollen (Ambrosia artemisiifolia). Pollen was exposed to acute O3 fumigation, with analysis of pollen viability, ROS and nitric oxide (NO) content, activity of nicotinamide adenine dinucleotide phosphate (NADP]H) oxidase, and expression of major allergens. There was decreased pollen viability after O3 fumigation, which indicates damage to the pollen membrane system, although the ROS and NO contents were not changed or were only slightly induced, respectively. Ozone exposure induced a significant enhancement of the ROS-generating enzyme NAD(P)H oxidase. The expression of the allergen Amb a 1 was not affected by O3, determined from the mRNA levels of the major allergens. We conclude that O3 can increase ragweed pollen allergenicity through stimulation of ROS-generating NAD(P)H oxidase. |
| Keywords: | NAD(P)H oxidase Ozone Pollen Ragweed ROS |
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