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A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples |
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| Authors: | Zhang Jiong Wan Yanjian Li Yuanyuan Zhang Qiongfang Xu Shunqing Zhu Huijun Shu Baihua |
| Institution: | a Minister of Education Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430030, PR China b Cranfield Health, Cranfield University, Kempston, Bedfordshire, MK43 0AL, United Kingdom |
| Abstract: | Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPARα-RXRα. It can be determined indirectly by immobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibody and subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα-RXRα probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L−1 with linear range between 2.5 ng L−1 and 75 ng L−1. Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS. |
| Keywords: | High-throughput Monitoring Quantum dots bioassay Perfluorooctane sulfonate |
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