| 辽河口沉积物中硝化细菌数量的时空变化分析 |
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| 引用本文： | 陈立广,樊景凤,关道明,赵化德,明红霞,吴利军,陈佳莹. 辽河口沉积物中硝化细菌数量的时空变化分析[J]. 海洋环境科学, 2010, 29(2) |
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| 作者姓名： | 陈立广 樊景凤 关道明 赵化德 明红霞 吴利军 陈佳莹 |
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| 作者单位： | 国家海洋环境监测中心,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023;国家海洋环境监测中心,辽宁,大连,116023 |
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| 基金项目： | 国家自然科学基金资助项目(40676060) |
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| 摘 要： | 采用MPN-Griess和MPN-PCR两种方法,于2007年春季(5月份)、夏季(8月份)和秋季(11月份)分3个航次,对辽河口区域沉积物中的硝化细菌进行了计数,并对硝化细菌在时间和空间上的分布进行了定量分析。结果表明,MPN-PCR方法要灵敏于MPN-Griess方法10~100倍,且重复性好,能够更快速、更准确的测定硝化细菌的数量;辽河口沉积物中硝化细菌的数量有明显的季节差异,分布趋势为春季最高、夏季最低;空间水平分布趋势为:从河口上游至深海区,硝化细菌数量逐渐降低。说明硝化细菌数量的时空分布与环境温度和沉积物中N的含量有关。
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| 关 键 词： | 辽河口 硝化细菌 MPN-Griess MPN-PCR 时空分布 |
Analysis of temporal and spatial distribution of nitrobacteria in sediment of Liaohe Estuary |
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| CHEN Li-guang,FAN Jing-feng,GUAN Dao-ming,ZHAO Hua-de,MING Hong-xia,WU Li-jun,CHEN Jia-ying. Analysis of temporal and spatial distribution of nitrobacteria in sediment of Liaohe Estuary[J]. Marine Environmental Science, 2010, 29(2) |
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| Authors: | CHEN Li-guang FAN Jing-feng GUAN Dao-ming ZHAO Hua-de MING Hong-xia WU Li-jun CHEN Jia-ying |
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| Affiliation: | 1.National Marine Environmental Monitoring Center;Dalian 116023;China;2.College of Life Science and Technology;Dalian Fisheries University;China |
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| Abstract: | Nitrobacteria in the sediment in Liaohe estuarine were investigated by MPN-Griess and MPN-PCR in spring(May),summer(Aug.) and autumn(Nov.) of 2007.The summarization of the results on temporal and spatial distribution of nitrobacteria in sediment was done.It is found that MPN-PCR is 10~100 times more sensitive than MPN-Griess and MPN-PCR characteristic of good repeatability,high test speed and accuracy in nitrobacteria test.The population of nitrobacteria in the sediment of Liaohe Estuary showed the obvious ... |
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| Keywords: | Liaohe Estuary nitrobacteria MPN-Griess MPN-PCR distribution |
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