Horizontal and vertical gene transfer drive sediment antibiotic resistome in an urban lagoon system |
| |
Authors: | Hongjie Wang Liyuan Hou Yongqin Liu Keshao Liu Lanping Zhang Fuyi Huang Lin Wang Azhar Rashi Anyi Hu Changping Yu |
| |
Affiliation: | 1. CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Department of Chemistry, SUNY College of Environmental Science and Forestry, Syracuse, NY 13210, USA;4. Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;5. Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;6. Nuclear Institute for Food and Agriculture, Tarnab, Peshawar 25000, Pakistan |
| |
Abstract: | Rapid urbanization has resulted in pervasive occurrence of antibiotic resistance genes (ARGs) in urban aquatic ecosystems. However, limited information is available concerning the ARG profiles and the forces responsible for their assembly in urban landscape lagoon systems. Here, we employed high-throughput quantitative PCR (HT-qPCR) to characterize the spatial variations of ARGs in surface and core sediments of Yundang Lagoon, China. The results indicated that the average richness and absolute abundance of ARGs were 11 and 53 times higher in the lagoon sediments as compared to pristine reference Tibetan lake sediments, highlighting the role of anthropogenic activities in ARG pollution. Co-occurrence network analysis indicated that various anaerobic prokaryotic genera belonging to Alpha-, Deltaproteobacteria, Bacteroidetes, Euryarchaeota, Firmicutes and Synergistetes were the potential hosts of ARGs. The partial least squares-path modeling (PLS-PM) analysis revealed positive and negative indirect effects of physicochemical factors and heavy metals on the lagoon ARG profiles, via biotic factors, respectively. The horizontal (mediated by mobile genetic elements) and vertical (mediated by prokaryotic communities) gene transfer may directly contribute the most to drive the abundance and composition of ARGs, respectively. Furthermore, the neutral community model demonstrated that the assembly of sediment ARG communities was jointly governed by deterministic and stochastic processes. Overall, this study provides novel insights into the diversity and distribution of ARGs in the benthic habitat of urban lagoon systems and underlying mechanisms for the spread and proliferation of ARGs. |
| |
Keywords: | Corresponding authors. Urban landscape lagoon Antibiotic resistance genes Heavy metals Horizontal and vertical gene transfer Assembly mechanisms High-throughput quantitative PCR |
|
| 点击此处可从《环境科学学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《环境科学学报(英文版)》下载全文 |
|