| 大凉疣螈脑源性神经营养因子(BDNF)基因的克隆与序列分析 |
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| 引用本文： | 曹玫,杨玉华,张义正. 大凉疣螈脑源性神经营养因子(BDNF)基因的克隆与序列分析[J]. 应用与环境生物学报, 2002, 8(3): 314-316 |
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| 作者姓名： | 曹玫 杨玉华 张义正 |
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| 作者单位： | 四川大学生命科学学院分子生物学实验室,成都,610064 |
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| 摘 要： | 大凉疣螈 (Ｔｙｌｏｔｏｔｒｉｔｏｎｔａｌｉａｎｇｅｎｓｉｓ)属于两栖纲有尾目蝾螈科 ,仅分布在我国四川省西南部 ,活动范围狭窄 ,数量有限 ,是我国二级保护动物[1] .两栖动物在生物进化中是承上启下的关键动物 ,它们开启了脊椎动物登上陆地的历史 ,而且是真正陆生脊椎动物—爬行动物的祖先 ,其生理、结构等方面与鱼类和爬行类都有着明显的差别 .脑源性神经营养因子 (ｂｒａｉｎ ｄｅｒｉｖｅｄｎｅｕｒｏｔｒｏｐｈｉｃｆａｃｔｏｒ,ＢＤＮＦ)是神经营养因子家族的一员 ,ＢＤＮＦ在脊椎动物胚胎神经系统的发育、分化及在成体神经系统维…
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| 关 键 词： | 大凉疣螈 脑源性神经营养因子 BDNF 基因克隆 序列分析 |
| 修稿时间： | 2001-08-28 |
CLONING AND SEQUENCE ANALYSIS OF BRAIN-DERIVED NEUROTROPHIC FACTOR GENE OF TYLOTOTRITON TALIANGENSIS |
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| CAO Mei YANG Yuhua , ZHANG Yizheng. CLONING AND SEQUENCE ANALYSIS OF BRAIN-DERIVED NEUROTROPHIC FACTOR GENE OF TYLOTOTRITON TALIANGENSIS[J]. Chinese Journal of Applied and Environmental Biology, 2002, 8(3): 314-316 |
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| Authors: | CAO Mei YANG Yuhua & ZHANG Yizheng |
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| Affiliation: | CAO Mei YANG Yuhua & ZHANG Yizheng * |
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| Abstract: | The full coding region of brain derived neurotrophic factor (BDNF) gene from Tylototriton taliangensis genome was amplified by PCR with the primers designed according to the sequence of human BDNF gene. Sequence analysis shows that the coding region has 744 bp in length and is highly homologous with that of mammals and aves. The homology of deduced BDNF protein sequence is about 90% with that of human. It is the first time to amplify and analyze BDNF gene from the newt genome, which will provide useful information for protecting and studying the severely endangered species. Fig1,Tab1,Ref 9 |
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| Keywords: | Tylototriton taliangensis brain derived neurotrophic factor (BDNF) PCR gene cloning sequence analysis |
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