生物除磷系统启动期聚磷菌的FISH原位分析与聚磷特性

应用FISH对以乙酸钠为碳源的强化生物除磷 (EBPR) SBR反应器启动期的微生物进行原位分析，考察除磷生态系统形成过程中聚磷菌种群结构、空间分布关系动态变化及其聚磷特性.结果表明，以异养菌为主的活性污泥经过厌氧/好氧驯化后，聚磷菌大量富集，在全菌中的比例由11.5%增加到40.48%.启动过程中，生物系统内菌群竞争持续进行：首先，聚磷菌淘汰异养菌，历时5 d；聚磷菌种群内选择过程历时19 d；经过优势聚磷菌群的二次增长后，共计34 d完成生物除磷系统的启动.富集过程中快速增殖的聚磷菌不能立刻行使除磷能力，要有一段“积累期”形成一定的PHA和poly-P储备.表现为污染物去除效率滞后于聚磷菌的增殖，经过4～8 d的 “积累期”后上升出现峰值.二次增长的优势聚磷菌群也经过“积累期”后才发挥作用.FISH图片显示，快速增殖期的聚磷菌菌体小，菌群结构松散.经过“积累期”之后，菌体不断增大，并开始紧密聚集形成致密的团状，此时反应器处理效率较高.

Characterization of Phosphate-Accumulating Organisms in Starting-up EBPR by FISH Analysis

Enhanced biological phosphorus removal(EBPR) process was operated in a laboratory-scale sequencing batch reactor(SBR) for one-month fed with acetate as the carbon source.The characteristic and the microbial population structure and space distribution dynamics of phosphate-accumulating organisms(PAOs) of start-up period were analyzed by fluorescent in situ hybridization(FISH).The relationship between enrichment of PAOs and phosphorus removal was discussed.PAOs could be enriched by recirculation activated slu...