| 谷氨酰胺转胺酶(MTG)分批发酵中温度对S.mobaraense生长及产酶影响的模型化 |
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| 引用本文： | 郑美英,堵国成,陈坚,CHEN Jian.谷氨酰胺转胺酶(MTG)分批发酵中温度对S.mobaraense生长及产酶影响的模型化[J].应用与环境生物学报,2000,6(5):462-467. |
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| 作者姓名： | 郑美英 堵国成 陈坚 CHEN Jian |
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| 作者单位： | 1. 无锡轻工大学生物工程学院环境生物技术研究室无锡 214036
2. Lab of Environmental Biotechnology, School of Biotechnology, Wuxi Univer sity of Light Industry, Wuxi, Jiangsu 214036 |
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| 基金项目： | 教育部优秀跨世纪人才培养计划 |
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| 摘 要： | 研究了2.5L小罐培养过程中控制温度为25℃～35℃时对细胞生长和MTG合成的影响.结果表明当控制相对较低的温度时，细胞生长的延滞期较长，当控制温度较高时，细胞生长的延滞期较短，达到最大DCW和最高MTG酶活的时间均较短；通过研究各种不同模型对细胞生长的影响得到最适合描述S.mobaraense生长与温度之间的关系方程为Schoolfield方程；通过对最大DCW和最大MTG酶活进行数学模拟，发现方程X(U)=-a
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| 关 键 词： | Streptoverticilliummobaraense 谷氨酰胺转胺酶 分批发酵 模型化研究 |
| 修稿时间： | 2000年4月18日 |
MODELING FOR EFFECT OF TEMPERATURE ON CELL GROWTH AND MTG PRO DUCTION IN BATCH MTG FERMENTATION WITH STREPTOVERTICILLIUM MOBARAENSE |
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| ZHENG Meiying,DU Guocheng,GUO Wangfang,CHEN Jian.MODELING FOR EFFECT OF TEMPERATURE ON CELL GROWTH AND MTG PRO DUCTION IN BATCH MTG FERMENTATION WITH STREPTOVERTICILLIUM MOBARAENSE[J].Chinese Journal of Applied and Environmental Biology,2000,6(5):462-467. |
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| Authors: | ZHENG Meiying DU Guocheng GUO Wangfang CHEN Jian |
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| Abstract: | Batch MTG fermentations by S. mobaraense W SH- Z2 at various temperatures ranging between 25℃～35℃ were studied. At 30℃, MTG activity and cell dry weight were higher than others, reached 2.94 u/mL and 25. 1 g/L, respectively. All kinds of models for effect of temperature on cell growt h were studi ed. The result showed that Schoolfield equation fit the experimental data fairly well. Eq. X(U)=-a0(θ-θ0)2+X1(U1)+a1｛1-exp［a 2*(θ-θ1)］｝ can describe the relationship between maximum of DCW (MT G ac tivity) and temperature. The lag time-temperature relation can be described by Eq. λ=l/(A0+A1*T+A1*T2+A2*T3+A )4*T4). The mode values of cell growth and MTG activity were com pared with experimental data, and the result showed that model simulation values and experimental data fit very well. Fig 6, Tab 6, Ref 18 |
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| Keywords: | Streptoverticillium mobaraense microbial transglutaminase batch fermentation modeling |
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