实验性糖尿病新西兰兔主要器官组织的病理学观察

将雄性新西兰兔随机分为:基础饲料组(即对照组:n=15)和高糖高脂饲料组(即实验组:n=15,喂食10％猪油,37％白蔗糖混合53％的基础饲料).共观察12周.于12周实验结束时,全部处死动物,取动物心脏、肝脏、胰腺、肾脏,用1O％福尔马林液固定,常规石蜡包埋切片,H、E染色,光学显微镜下观察并照相.结果表明:动物心肌细胞肥大,并出现灶性坏死,淋巴细胞浸润,心肌细胞间成纤维细胞增生和毛细血管增生,胶原纤维增加;肝细胞有明显脂肪变性,细胞肿胀;肾脏表现有肾小球球囊增大,细胞增多,毛细血管壁增厚,肾小管上皮细胞胞体增大;胰腺组织表现为胰岛分布稀疏,数量减少,胰岛萎缩,胰岛细胞数量减少,成纤维细胞增生.

Pathological Observations of Main Organs and Tissues in New Zealand White Rabbits with Experimental Diabetes

Male New Zealand White rabbits were divided into two groups: normal fodder group (n=15) and high-sugar and high fat fodder group (n=15, with 10% lard, 37% white cane sugar and 53% normal fodder), and observed for 12 weeks. At the end of 12 weeks all the rabbits were killed to get the hearts, livers, kidneys and pancreas, which were fixed with 10% formal in sectioned with regular paraffin wrapping. Stained with H.E, and then observed with microscopes. The results show that 12 weeks later the cardiac muscle cells in the rabbits of the experimental group were loose and had necrosis of infection, and they're some lymphocyte infiltration. Fibroblast and blood capillary were of hyperplasic in the cardiac muscle cells. Collagen increased. The liver cells developed clear statuses and Swelling. In the kidneys the glomeruli became larger, the cell number increased, the walls of capillary vessel became thicker and theelicited in the urinitubube became larger. The tissue structures in the pancreas developed obvious pathological changes. The pancreas islets were atrophied, there was a decrease of cell's amount in pancreas islets and fibroblast was of hvperplasic. There ware a few lymphocyte infiltrations around pancreas islets.