| 零价纳米铁对大肠杆菌的毒性效应 |
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| 引用本文： | 王学,李勇超,李铁龙,金朝晖. 零价纳米铁对大肠杆菌的毒性效应[J]. 生态毒理学报, 2012, 7(1): 49-56 |
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| 作者姓名： | 王学 李勇超 李铁龙 金朝晖 |
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| 作者单位： | 1. 南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室,天津,300071
2. 南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室,天津300071;天津市城市生态环境修复与污染防治重点实验室,天津300071 |
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| 基金项目： | 国家青年科学基金项目(20907023)；国家自然科学基金项目 (40971254) |
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| 摘 要： | 以大肠杆菌为研究对象,通过检测尺寸为20 nm的零价纳米铁暴露下大肠杆菌形貌、生长曲线和细胞内酶活性的变化,研究了零价纳米铁对大肠杆菌的毒性效应,并探讨了其可能的毒性机制.用透射电镜(TEM)观察零价纳米铁与大肠杆菌(JM109)接触后细胞的形态变化；用0、112、560和1 120 mg·L-1的零价纳米铁染毒大肠杆...
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| 关 键 词： | 零价纳米铁 大肠杆菌 生长曲线 酶活性 氧化损伤 |
| 收稿时间： | 2011-07-21 |
| 修稿时间： | 2011-09-19 |
Toxicity Effects of Nano-Fe0 on Escherichia coli |
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| Wang Xue,Li Yongchao,Li Tielong and Jin Zhaohui. Toxicity Effects of Nano-Fe0 on Escherichia coli[J]. Asian Journal of Ecotoxicology, 2012, 7(1): 49-56 |
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| Authors: | Wang Xue Li Yongchao Li Tielong Jin Zhaohui |
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| Affiliation: | Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education, College of Environmental Science and Engineering, Nankai University,Tianjin 300071,China;Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education, College of Environmental Science and Engineering, Nankai University,Tianjin 300071,China;1. Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education, College of Environmental Science and Engineering, Nankai University,Tianjin 300071,China2. Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China;1. Key Laboratory of Pollution Processes and Environmental Criteria of Ministry of Education, College of Environmental Science and Engineering, Nankai University,Tianjin 300071,China2. Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China |
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| Abstract: | Cytotoxicity effects and mechanism of 20 nm nano-Fe0 on Escherichia coli were studied. In this paper, morphology of E. coli in the presence of nano-Fe0 was observed by transmission electron microscopy (TEM). E.coli cells were exposed to 0, 112, 560, 1 120 mg·L-1 nano-Fe0 particles. Experiments were carried out to study the inhibitive effect of nano-Fe0 on E.coli growth. E. coli exposed to nano-Fe0 for 24 h, the activities of lactate dehydrogenase (LDH), cellular superoxide dismutase (SOD), malondialdehyde (MDA) were determined. Moreover, the influence of addition of N-Acetylcysteine (NAC) on the viability of cells was studied. TEM images of E.coli cells treated with nano-Fe0 exhibited significant disruption of the cell membranes. Experimental results of growth curves showed that nano-Fe0 nanoparticles could inhibit the growth of cells in liquid LB medium. Additionally, the shortened log phase and the prolonged stable phase of E.coli both resulted from the toxicity of nano-Fe0. Furthermore, the stable phase of E.coli is proportional to the concentration of nano-Fe0 particles. The activities of LDH and the levels of MDA significantly increased and there was a linear relationship between the concentration of nano-Fe0 and MDA levels, while the activities of SOD significantly decreased. To nano-Fe0-NAC exposure groups, the number of cell was higher than that of simple nano-Fe0 exposure groups. The results demonstrated that oxidative damage was the mechanism of nano-Fe0 toxicity to E. coli. |
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| Keywords: | nano-Fe0 Escherichia coli growth curves enzymes activities oxidative damage |
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