Peroxyacetyl Nitrate-Induced Inhibition of Cell Wall Metabolism

Levels of PAN which inhibit oat coleoptile section growth reversibly, severely inhibit metabolism of cellulose and of cell wall noncellulosic polysaccharides. An enzyme in the coleoptile cell walls which hydrolyzes some of the noncellulosic glucan was partially inactivated by direct PAN treatment of homogenized cell wall. Treatment of intact cells by PAN also resulted in a partially inactivated enzyme. Coleoptile phos-phoglucomutase was partially inactivated by treatment with PAN both in vitro and in vivo. After treatment of intact cells with PAN, phosphoglucomutase associated with subcellular particles was more severely inactivated than was soluble enzyme. Uridine diphosphate glucose pyrophosphorylase from coleoptiles was inactivated by PAN in vitro but not in vivo. A particulate cellulose synthetase from coleoptiles was inactivated by PAN both in vitro and in vivo. Since cell wall biosynthesis and or degradation are needed for expansion it is concluded that PAN inhibition of these enzymes may account for reduced cell growth. The work of Dr. Morris J. Garber who carried out the analysis of variance is gratefully acknowledged.