养殖废水处理系统中微生物菌群结构及动态变化

采用高通量测序技术对养猪场废水处理系统的细菌群落组成进行分析，同时对细菌群落、污水水质和抗生素含量相关性进行分析.研究结果表明，污水各处理单元中的菌群结构存在显著差异，且细菌菌群结构随处理进程逐步改变，其中集污池与厌氧反应器的细菌菌群优势门类为Firmicutes、Bacteroidetes和Proteobacteria，曝气池的细菌菌群优势门类变为Firmicutes、Verrucomicrobia、Bacteroidetes、Proteobacteria和Candidatus Saccharibacteria，氧化塘的细菌菌群优势门类为Firmicutes、Proteobacteria、Chloroflexi、Euryarchaeota和Bacteroidetes.相关性分析结果表明，污水中的细菌群落与水质环境因子之间存在显著相关（P<0.05）.其中，种属Acinetobacter、Bacteroides、Prevotella、Lachnospiracea_incertae_sedis与COD、TN、NH₄⁺-N呈显著正相关，Clostridium sensu strict、Methanothrix与TN、NH₄⁺-N呈显著负相关，Petrimonas、Lachnospiracea_incertae_sedis、Clostridium XlVa、Tissierella与磺胺甲恶唑、磺胺间甲氧嘧啶、强力霉素、土霉素呈极显著正相关，Acinetobacter、Bacteroides、Prevotella与恩诺沙星、四环素呈显著正相关，Prevotella、Lachnospiracea_incertae_sedis与环丙沙星呈显著正相关，Clostridium sensu stricto、Petrimonas与氧氟沙星呈显著正相关.

Microbial community structure and dynamics in swine wastewater treatment system

To elucidate the bacterial community dynamics in swine wastewater treatment system, the bacterial community characteristics in different treatment units of swine wastewater was analyzed by Illumina Miseq high-throughput sequencing technologies, and the correlation between bacterial community and water quality or antibiotics was investigated. The results demonstrated that microbial community structure varies greatly in different wastewater treatment processes. The dominant bacteria in fouling pool and anaerobic reator were Firmicutes, Bacteroidetes and Proteobacteria, the dominant bacteria in aeration tan were Firmicutes, Verrucomicrobia, Bacteroidetes, Proteobacteria and Candidatus Saccharibacteria, while in oxidation pond, the dominant bacteria were Firmicutes, Proteobacteria, Chloroflexi, Euryarchaeota and Bacteroidetes. The microbial comminity structure in wasterwater treatment system was significantly correlated with both water quality and antibiotics (P<0.05). Acinetobacter, Bacteroides, Prevotella, Lachnospiracea_incertae_sedis correlated closely with COD、TN、NH₄⁺-N. Clostridium sensu strict, Methanothrix correlated closely with TN、NH₄⁺-N. Petrimonas, Lachnospiracea_incertae_sedis, Clostridium XlVa, Tissierella extremely significant positively correlated with sulfamethoxazole, sulfamethoxazole, doxycycline, oxytetracycline. Acinetobacter, Bacteroides, Prevotella significant positively correlated with enrofloxacin and tetracycline. Prevotella, Lachnospiracea_incertae_sedis positively correlated with ciprofloxacin. Clostridium sensu stricto, Petrimonas positively correlated ofloxacin.