Mitigation of peroxidative stress for barley exposed to cadmium in the presence of water-extractable organic matter from compost-like materials |
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Authors: | Naoya Tachibana Kenya Nagasawa Binhui Wang Kazuki Nishiya Masami Fukushima Hikari Kanno Takuro Shinano Keiki Okazaki |
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Affiliation: | 1. Laboratory of Chemical Resources, Division of Sustainable Resources Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan;2. Daiso KET Institute Corporation, 9-joudori 2-chome 2346-1, Asahikawa 070-0039, Japan;3. National Agriculture and Food Research Organization (NARO), Hokkaido Agricultural Research Center (HARC), Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan |
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Abstract: | The effects of water-extractable organic matter (WEOM) from compost-like materials on peroxidative stress were investigated for hydroponic culture of barley exposed to Cd. In the presence of WEOM, lipoxygenase activity and malondialdehyde, indices of peroxidative stress in barley, were significantly reduced, compared to those with Cd alone (5 μM) for a 30-d culture (p < 0.05). In addition, Cd uptake in the presence of WEOM samples was significantly lower than that in their absence (p < 0.05). These results indicate that the addition of WEOM can be effective in mitigating the peroxidative stress in barley exposed to Cd. Of the total Cd in the solution, 7–8% was complexed with WEOM, indicating that the complexation of Cd with WEOM is a minor factor in reducing Cd-induced stress in barley. The WEOM sample was purified by cation-exchange column and ultrafiltration to remove the nutrient minerals, such as Ca, Mg and Fe. When the purified WEOM was employed for hydroponic culture in the presence of Cd, significant decreases in peroxidative stress and Cd uptake were observed (p < 0.05). These results show that the organic components in WEOM contribute to the mitigation of peroxidative stress in barley exposed to Cd. |
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Keywords: | Water-extractable organic matter Compost-like materials Barley Malondialdehyde Lipoxygenase Cadmium |
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