Abstract: | Nitrogen (N) losses from agricultural lands in the Midwest United States are contributing to the expansion of the hypoxic zone in the Gulf of Mexico. This study evaluated the importance of inter‐annual variability in precipitation, land cover, and N fertilizer use on NO3 + NO2‐N loads in seven United States Midwestern Rivers using the backward stepwise regression analysis. At the annual scale, fluctuations in the current and previous years’ precipitations explained much of the variation in streamflow, baseflow, and N‐load. Previous years precipitation effects were associated with fillable soil porosity. In some years, higher residual soil N from previous dry years also contributed to an increase in N‐load. Area under soybean production (SOY), a surrogate for replacement of prairies and small grains was generally not a significant explanatory variable. Fertilizer use from 1987 to 2012 was also not a significant explanatory variable in the annual analysis. Precipitation in both the current and previous months and previous year were important in explaining variation in monthly streamflow, baseflow, and N‐load. SOY was significant in one or two months from June to August, but had a higher p‐value than precipitation. We conclude recent increases in river N‐loads are primarily due to wet climate and minimally due to the changes in land cover or N fertilizer use. Under current cropping systems and agronomic N application rates, tile water remediation will be necessary to reduce river N‐loads. |