| DNA-benzoquinone adducts analyzed by nuclease P1 m ediated 32P-postlabeling method |
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| 作者姓名： | Jiang Xiangning Liu Sufen Xu Xiaobai |
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| 作者单位： | Research Center for Eco-Environmental Sciences，Chinese Academy of Sciences，Beijing 100085，China,Research Center for Eco-Environmental Sciences，Chinese Academy of Sciences，Beijing 100085，China,Research Center for Eco-Environmental Sciences，Chinese Academy of Sciences，Beijing 100085，China |
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| 摘 要： | With the super high sensitivity，high reproducibility and accumcy，nuclease P1 mediated 32P-postlabeling version has been successfully used to analyze the DNA-benzoquinone(DNA-BQ)adducts formed from m vitro cultured cells，reaction of benzoquinone ith calf thymus DNA and nucleoside monophosphates．It has been proven that the maior radioactive spot，contributing more than 70% of the total radioactivity of DNA-BQ adducts detected，is from deoxycytidine(dC)modified by benzoqumone while a minor one from deoxyguanosme(dGl．The method is capable of detecting 1 adduct in 109 to 1010 DNA bases．
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| 关 键 词： | P1 mediated 32p-postlabelmg；DNA-BQ adducts；dC-BQ adduct；dG-BQ adduct |
| 收稿时间： | 1991-09-16 |
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