| 用酶联免疫吸附法测定鱼类体内金属硫蛋白 |
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| 引用本文： | 林芃,任宏伟,茹炳根.用酶联免疫吸附法测定鱼类体内金属硫蛋白[J].环境污染与防治,2001,23(5):265-267. |
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| 作者姓名： | 林芃 任宏伟 茹炳根 |
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| 作者单位： | 1. 大连大学化学与化学工程系,
2. 北京大学生命科学学院 |
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| 摘 要： | 用酶联免疫吸附法测定鱼体内（肝，肾）MT。鱼组织经匀浆、加热、离心、将上清液以包被液稀释后，与一抗、二抗反应，求得MT含量。与镉／血红蛋白饱和法比较、本法显示了特异、简便、及灵敏的特点。同时，用酶联免疫吸附法测定了取自武汉东湖不同水域的鱼类、不同种类的鱼类的组织脏器的MT的含量，用以研究水体质量。
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| 关 键 词： | 金属硫蛋白 酶联免疫吸附法 特异性 鱼类体内 生物监测 |
Study for quantitative determination of Metallothionein with enzyme linked immunosorbent assay(ELISA) |
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| Lin Peng,et al..Study for quantitative determination of Metallothionein with enzyme linked immunosorbent assay(ELISA)[J].Environmental Pollution & Control,2001,23(5):265-267. |
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| Authors: | Lin Peng |
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| Institution: | Lin Peng,et al. Department of Chemistry and Chemical Engineering,Dalian University,Dalian 115522 |
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| Abstract: | Determination of metallothionein of fish tissues(liver and kidney) has been established. Fish tissues were homogenized, heated and centrifuged. The supernatent was used to react with polyclonal\|antibody. Comparing with the cadmium/hemoglobin saturation method, this method was shown distinctive, credible and sensitive. |
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| Keywords: | Metallothionein\ Enzyme linked immunosorbent assay\ Spicific\ Sensitivity |
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