藻菌固定化在畜禽养殖废水深度处理中的参数优化

以模拟废水为研究对象，基于藻菌包埋固定化在畜禽养殖废水处理中的处理效果和微生物生长情况，来优化藻菌固定化小球的制备条件和试验参数。以固定化小球强度、微生物生长情况、污染物去除效果为考察指标，通过正交试验，采用多指标全概率分析法和SPSS软件分析，确定藻菌固定化的优选参数组合:海藻酸钠（SA）、氯化钙（CaCl₂）浓度及固定化时间。然后，运用Design-Expert 10软件设计响应曲面实验，对相关实验参数做进一步优化。实验结果表明:正交实验得到的一组优选参数为SA浓度3%、CaCl₂浓度1%、固定化时间12 h；最终通过响应曲面法进一步优化得到最优参数为SA浓度4%、CaCl₂浓度0.5%、固定化时间6 h，在此最优参数条件下固定化小球强度、微藻生长速率、TN去除率分别达到-13.58%、 0.193、 89.55%。

OPTIMIZATION OF PARAMETERS IN ADVANCED TREATMENT OF LIVESTOCK WASTEWATER BY ALGAL-BACTERIA IMMOBILIZATION

Artificial wastewater was used in this study, and parameters of the algae-bacteria immobilized pellets and the growth of microorganism were optimized based on treatment efficiency of livestock wastewater. The orthogonal experiment was designed for optimization of immobilized pellet strength, the growth of microorganism and removal efficiency, using multi-index full probability analysis and SPSS software analysis. The results were concluded as a set of parameters including the concentration of sodium alginate(SA) and calcium chloride(CaCl₂) and the immobilization time. Then, the response surface experiment designed by analysis software Design-Expert 10 were carried out to further optimize these parameters. The results showed that the optimized parameters of orthogonal experiment were SA concentration of 3%, the CaCl₂ concentration of 1%, and the immobilization time of 12 hours; finally, the optimal parameters obtained by response surface methodology were:SA concentration of 4%, CaCl₂ concentration of 0.5%, and immobilization time of 6 hours; under the optimal condition, the immobilized pellets strength, microalgae growth rate, TN removal rate reached-13.58%, 0.193 and 89.55%, respectively.