臭氧高级氧化含酚类废水出水的急性生物毒性和荧光光谱特征分析

研究了苯酚、间甲酚、邻甲酚和间苯二酚等酚类化合物在臭氧氧化过程中生物毒性的变化，揭示了反应中高毒性中间产物的荧光光谱特征。研究发现:4种酚类化合物被臭氧氧化后，生物毒性均呈先增加后减弱的趋势，其中臭氧氧化苯酚、间甲酚、邻甲酚和间苯二酚的生物毒性最大值分别是其初始毒性的16.7，26.3，34.8，3.2倍。利用Na₂SO₃还原臭氧氧化出水，急性生物毒性可得到高效去除，4种污染物毒性单位最高点的去除率分别为95.2%、94.5%、87.3%和44.4%，并且毒性单位的变化量和在295~305 nm激发波长下的荧光发射光谱峰强度的恢复量呈显著相关（P<0.05），可为构建基于光学特征的臭氧氧化出水中生物毒性的快速定量表征方法提供新思路。

CHANGES OF ACUTE TOXICITY AND FLUORESCENCE SPECTRUM PROPERTIES OF PHENOL WASTEWATER TREATED BY OZONE ADVANCED OXIDATION

In this study, the changes of biological toxicity during the ozonation of phenol, m-cresol, o-cresol and m-dihydroxybenzene were investigated, and the fluorescence spectra of highly toxic intermediates were revealed. It was found that the biological toxicity of four phenolic compounds firstly increased and then decreased after ozonation with the reaction time passed. The maximum biological toxicity of phenol, m-cresol, o-cresol and m-dihydroxybenzene was 16.7, 26.3, 34.8, 3.2 times of their initial toxicity, respectively. At the toxicity peaks, the toxic unit were removed by 95.2%, 94.5%, 87.3% and 44.4% respectively, after adding Na₂SO₃, and there was a significant correlation between the change of toxic unit and the recovery of fluorescence intensity peak at emission wavelength of 295~305 nm (P<0.05). It was speculated that the highly toxic intermediates formed during the ozonation of phenols were mainly oxidative carbonyl compounds. This study could provide new insight into the construction of rapid quantitative characterization of biological toxicity after ozonation based on the optical characteristics.