Application of random amplified polymorphic DNA (RAPD) markers to evaluate intraspecific genetic variation in red mullet (Mullus barbatus) |
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Authors: | Z Mamuris A P Apostolidis A J Theodorou C Triantaphyllidis |
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Institution: | (1) Department of Applied and Theoretical Sciences, University of Thessaly, Pedion Areos 38334, Volos, Greece, GR;(2) Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54006, Thessaloniki, Macedonia, Greece, GR;(3) Laboratory of Oceanography, Department of Agriculture Crop and Animal Production, University of Thessaly, Pedion Areos 38334, Volos, Greece, GR |
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Abstract: | The random amplified polymorphic DNA (RAPD) technique was used to evaluate genetic affinities among eight red mullet (Mullus barbatus L., 1758) samples from the Mediterranean Sea. Twenty-nine random primers were used. Despite the variability which was found
within samples, no specific RAPD marker for the discrimination of the populations was detected. The data analysis revealed
that the genetic diversity among populations is positively related to their geographic distances. The results of this study
indicate that the estimated intraspecific variation may be more␣pronounced with RAPD markers than with allozymes when the
two approaches are applied on the same populations.
Received: 7 November 1997 / Accepted: 30 May 1998 |
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