淡水鱼脑AChE的动力学特性及抗抑制性

用PEG2000双水相萃取、DEAE-Sephadex A-50和SephadexG-200方法分离提纯麦穗鱼(Pseudorasbora parva)、金鱼(Carassius auratus)和虹鳟鱼(Salmo gairdneri)脑乙酰胆碱酯酶(AChE),比较这3种淡水鱼脑AChE纯酶液与粗酶液的动力学特性、抗抑制性及其种类之间的差异.结果表明,这3种鱼脑AChE的性质在种类间的差异要远远小于其在粗酶状态时酶活性的差异,由此得出AChE性质在种类间的差异并非影响这3种淡水鱼脑AChE活性差异的主要因素;由AChE抗抑制试验结果可知,这3种鱼脑AChE粗酶液对经溴水氧化后的马拉硫磷和三唑硫磷的敏感性及种间的差异都远大于纯酶液.

Kinetic characters and inhibition resistibility of brain AChE of freshwater fish

Acetylcholinesterase (AChE) from the brain of topmouth gudgeon (Pseudorasbora parva), goldfish (Carassius auratus) and rainbow trout (Oncorrhychus mykiss, formerly named Salmo gairdneri) was purified with PEG2000 two liquor phases extraction, DEAE-Sephadex A-50 and Sephadex G-200 techniques. Kinetic characters, inhibition resistibility and difference between species of crude and purified enzymes of three fresh-water fishes were compared. Among these three fishes the difference of AChE property was far less than the difference of the crude enzyme activity; thus, the property difference was not the main factor influencing the activity difference. The sensitivity to malathion and triazopos and the species difference of crude enzyme liquor were far more than those of the liquor treated with bromine water.