| 麻黄愈伤组织细胞的悬浮培养 |
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| 引用本文： | 曹有龙,许兴,赵军,陈放. 麻黄愈伤组织细胞的悬浮培养[J]. 应用与环境生物学报, 2000, 6(1): 36-38 |
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| 作者姓名： | 曹有龙 许兴 赵军 陈放 |
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| 作者单位： | 1. 四川大学生物系,成都,610064
2. 宁夏农科院农业生物技术中心重点实验室,银川,750002 |
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| 基金项目： | 宁夏回族自治区科委资助项目 |
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| 摘 要： | 用中麻黄幼苗的３种外植体进行了离体培养。不同外植体诱导愈伤组织的结果表明：最佳培养基为ＭＳ＋２ｍｇ／Ｌ２，４－Ｄ＋１ｍｇ／Ｌ６－ＢＡ；最佳外植体为下胚轴；用源于下胚轴的愈伤组织进行悬浮培养，从第３代开始建立起稳定的悬浮系。愈伤组织、悬浮培养细胞及培养液中都含有麻黄碱，悬浮培养液中的麻黄碱含量高于愈伤组织和悬浮培养细胞。表２参１２
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| 关 键 词： | 麻黄 愈伤组织 悬浮培养 细胞培养 药用作物 |
| 修稿时间： | 1998-11-03 |
CELL SUSPENSION CULTURE OF EPHEDRA INTERMEDIA SCHRENKET |
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| CAO Youlong,XU Xing,ZHAO Jun,CHEN Fang. CELL SUSPENSION CULTURE OF EPHEDRA INTERMEDIA SCHRENKET[J]. Chinese Journal of Applied and Environmental Biology, 2000, 6(1): 36-38 |
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| Authors: | CAO Youlong XU Xing ZHAO Jun CHEN Fang |
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| Abstract: | Differentexplants of Ephedra intermedia Schrenket seedling were cultured on the media of differentplant hormone combinations. The results indicated that MS+2 mg/L 2,4-D+1 mg/L 6-BA mediumwas the best to induce callus, with the highest induction frequency from hypocotyl. Thecalli from hypocotyl were cultured in liquid medium and stable suspension culture systemwas established after 3 successive transfer cultures. Alkaloid components were found inthe calli induced from hypocotyl, suspension cells and liquid culture medium. The contentof ephedine in liquid medium was higher than those in calli and suspension cells. Tab 2,Ref 12 |
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| Keywords: | Ephedra intermedia callus suspension culture |
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