Evaluation of potential genotoxic/cytotoxic effects induced by epoxiconazole and fenpropimorph-based fungicide in bovine lymphocytes in vitro |
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Authors: | Monika Drážovská Katarína Šiviková Beáta Holečková Ján Dianovský Martina Galdíková Viera Schwarzbacherová |
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Affiliation: | 1. Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy in Ko?ice, Ko?ice, Slovak Republicdrazovska@gmail.com;3. Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy in Ko?ice, Ko?ice, Slovak Republic |
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Abstract: | Potential genotoxic/cytotoxic effects of the epoxiconazole/fenpropimorph-based fungicide were investigated using single cell gel electrophoresis and cytogenetic assays: chromosomal aberrations, sister chromatid exchanges, micronuclei and fluorescence in situ hybridization in cultured bovine lymphocytes. No statistically significant elevations of DNA damage and increases in cytogenetic endpoints were seen. However, evident cytotoxic effect presented as a decrease in mitotic and proliferation indices were recorded after exposure of bovine lymphocytes to the fungicide for 24 and 48 h at concentrations ranging from 3 to 15 µg mL?1 (P < 0.05, P < 0.01, P < 0.001). Similarly, for 24 h an inhibition in the cytokinesis block proliferation index (CBPI) was obtained after exposure to the fungicide at concentrations ranging from 1.5 to 15 µg mL?1 (P < 0.01, P < 0.001) in each donor. |
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Keywords: | Conazoles chromosomal aberrations genotoxic/cytotoxic effect micronucleus single cell gel electrophoresis sister chromatid exchanges |
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