| 水稻OsGPCR基因的克隆及遗传转化分析 |
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| 引用本文： | 周宇科,牛向丽,刘永胜.水稻OsGPCR基因的克隆及遗传转化分析[J].应用与环境生物学报,2011(1):7-11. |
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| 作者姓名： | 周宇科 牛向丽 刘永胜 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室;重庆大学农学及生命科学研究院; |
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| 基金项目： | 国家杰出青年科学基金项目(No.30825030); 国家转基因生物新品种培育科技重大专项课题(Nos.2009ZX08001-011B,2009ZX08009-072B)资助~~ |
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| 摘 要： | G蛋白偶联受体(G protein-coupled receptors,GPCR)基因家族是目前已知最大的膜受体超家族,参与生物的生长发育调控和激素应答.通过RT-PCR分离了水稻中一个具有7次跨膜(Seven transmembrane,7TM)结构域的OsGPCR候选基因;分析该基因启动子序列,发现它含有赤霉素GA应答相关元件.为分析其功能,分别构建OsGPCR过表达和RNA干涉载体,并通过农杆菌介导法转化获得转基因水稻植株.实验结果表明,较之野生型,过表达转基因植株对赤霉素反应更敏感,RNA干涉植株则反之.
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| 关 键 词： | 水稻G蛋白偶联受体 7次跨膜结构域 农杆菌介导转化 赤霉素 转基因水稻 |
Cloning and Genetic Transformation of Rice OsGPCR Gene |
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| ZHOU Yuke,NIU Xiangli & LIU Yongsheng.Cloning and Genetic Transformation of Rice OsGPCR Gene[J].Chinese Journal of Applied and Environmental Biology,2011(1):7-11. |
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| Authors: | ZHOU Yuke NIU Xiangli & LIU Yongsheng |
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| Institution: | ZHOU Yuke1,NIU Xiangli2 & LIU Yongsheng1(1Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China)(2Institute of Agricultural and Life Sciences,Chongqing University,Chongqing 400030,China) |
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| Abstract: | G protein-coupled receptors consist of the largest plasma membrane-spanning superfamily known at present,of which the members participate in development regulation and hormone response of organisms.In the present study,a seven-transmembrane(7TM) domain containing candidate GPCR gene was isolated from Oryza sative L.Nipponbare by RT-PCR.The analysis of the upstream sequence suggested that OsGPCR might be involved in gibberellin(GA) signaling pathway.To analyze the function of OsGPCR,over-expression and RNA i... |
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| Keywords: | OsGPCR seven transmembrane domain Agrobacterium-mediated transformation gibberellin transgenic rice |
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