HPLC-ICP-MS在植物有机硒形态分析中的应用现状

植物是人们摄取硒的主要来源,富硒植物中富含可开发利用的有机硒,我国湖北恩施高硒地区富硒植物资源丰富亟待开发利用。植物中硒的形态分析是研究开发富硒植物资源的基础,而高效液相色谱与电感耦合等离子体质谱联用技术(HPLC-ICP-MS)是目前硒形态分析使用最为广泛的技术,它在样品的前处理、分析灵敏度、准确性等方面均具有优势,可以实现富硒植物中含硒生物大分子、硒形态以及与氨基酸结合位点及机理的分析。因此作者综述了植物样品的预处理过程,介绍了HPLC-ICP-MS联用技术在测定植物硒形态及其应用方面的研究成果,为我国富硒植物、富硒产品中硒形态的分析测定、开发富硒植物资源提供指导。

An Application of HPLC-ICP-MS to Analyzing Selenium Species in Plants

A plant is the main source of selenium which humans take in, and Se-enriched plants contain large amounts of organic Se which can be developed and applied for human health. There is an abundant seleniferous plant resource in the Enshi area, Hubei Province, China, which urgently needs development and utilization. Speciation analysis of selenium in plants is the basis for the development of Se-enriched plants. HPLC-ICP-MS is the most widely used technology of Se species analysis and has much more advantages in the sample pretreatment process, sensitivity, accuracy and so on. It can perform the analysis of the macromolecules containing selenium, and selenoamino acids binding sites and help to understand the mechanism of Se accumulation. This paper reviewed the study results of analysis of Se species in plants, particularly, and introduced the application of HPLC-ICP-MS in determination of Se speciation both at home and abroad. This could provide the guidance of Se species determination for the development of Se-enriched plant resources in China.