一株好氧反硝化细菌的鉴定及其在废水处理中的应用

从武汉市郊稻田土壤中分离得到一株好氧反硝化细菌HS-03,对其反硝化能力进行了研究.结果表明:细菌HS-03在好氧条件下能有效去除人工废水中的NO3-(10mmol/L),去除率达90%以上,并且在反应过程中没有NO2-的积累.菌株HS-03与Pseudomonasstutzeri的16SrDNA序列具有99.1%的相似性.对该菌株的16SrDNA序列以及生理生化特征分析表明,细菌HS-03是P.stutzeri的一个新分离种.实验采用ClastalW和PHYLIP程序对该菌株与报道菌进行了系统发育进化分析.图5表2参14

IDENTIFICATION OF AN AEROBIC DENITRIFYING BACTERIUM AND ITS POTENTIAL APPLICATION IN WASTEWATER TREATMENT

A bacterium strain HS-03 isolated from farm active sludge in Wuhan was found capable of aerobic denitrification. The effect of different inoculated amounts of pre-culture was analyzed. The denitrifying rates of this strain under aerobic and micro-anaerobic conditions were compared. The result indicated that the rate was almost not affected by the presence of oxygen. More than 90% of 10 mmol/L nitrate was removed within 36 hours by this bacterium in the specific medium. During denitrifying process, nitrite was always kept at very low level. The denitrification potential of this bacterium was evaluated for treating artificial wastewater under fully aerobic conditions. More than 90% of 10 mmol/L nitrate in the wastewater was removed when it co-denitrified with fungi Aspergillus oryzae. The main biochemical and physiological features of this strain were characterized. The 16S rDNA sequence was compared with the published data in GenBank by using BLAST. The BLAST level of similarity to Pseudomonas stutzeri is 99.1%. These results proved that strain HS-03 was a new strain belonging to P. stutzeri. Fig 5, Tab 2, Ref 14