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Banding pattern of mussel (Mytilus galloprovincialis) chromosomes induced by 2 × SSC/Giemsa-stain treatment
Authors:J Méndez  J J Pasantes  M J Martínez-Expósito
Institution:(1) Departamento de Biología Celular y Molecular, Área de Genética, Universidad de La Coruña, E-15071 La Coruña, Spain
Abstract:Mytilus galloprovincialis were collected from an intertidal population in NW Spain in 1988, and chromosomes from the gill tissue of 37 individuals were studied. The present paper describes the banding pattern of aM. galloprovincialis population which enabled us to identify all pairs of chromosomes. Banding was induced by means of a 2 × SSC (0.3M sodium chloride:0.03M sodium citrate)/Giemsa-staining technique, and a diagrammatic representation was constructed based on mean number of bands. The application of this banding technique may prove useful in future research related to the cytogenetics and cytotaxonomy of mussels and other molluscs.
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