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两株产电菌电子介体介导的胞外电子传递机理研究
引用本文:牛雨薇,袁林江,刘冰茵,王茹. 两株产电菌电子介体介导的胞外电子传递机理研究[J]. 中国环境科学, 2021, 40(11): 4911-4918
作者姓名:牛雨薇  袁林江  刘冰茵  王茹
作者单位:西安建筑科技大学, 陕西省环境工程重点实验室, 教育部西北水资源和环境生态重点实验室, 陕西 西安 710055
基金项目:重大水专项(2009ZX07212-002);国家自然科学科学基金重大项目(51808433)
摘    要:构建了Klebsiella oxytoca d7和Shewanella sp.F1 2种纯菌燃料电池,探究了产电菌在产电过程中电子介体传递电子机制.结果表明:K.oxytoca d7只有作产电呼吸时,才会产生电子介体,而Shewanella. sp.F1在好氧呼吸、厌氧呼吸、产电呼吸下均能产生介电体,说明电子介体的产生与产电菌种类有关.两种菌介电体氧化还原电位相当(-250,210mV),且介于呼吸链NADH和辅酶Q之间.其介电体在胞内呼吸链上截获的电子均源于NADH,说明电子“逸出”位点只取决于介电体本身.K.oxytoca d7在碳源充足和不足时,电子介体产电量分别占总电量的60%和41%;Shewanella. sp.F1分别是57%和50%,说明在碳源充足时,2种菌的产电呼吸都以电子介体机制为主.介电体传递电子过程直接影响了阳极底物的转化和燃料电池的产电性能.

关 键 词:产电呼吸  电子介体  产电菌  微生物燃料电池  

Comparative study of extracellular electron transfer mechanism mediated by electron transfer mediators in two strains of electricigenic bacteria
NIU Yu-wei,YUAN Lin-jiang,LIU Bing-yin,WANG Ru. Comparative study of extracellular electron transfer mechanism mediated by electron transfer mediators in two strains of electricigenic bacteria[J]. China Environmental Science, 2021, 40(11): 4911-4918
Authors:NIU Yu-wei  YUAN Lin-jiang  LIU Bing-yin  WANG Ru
Affiliation:Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
Abstract:Microbial fuel cells (MFCs) inoculated pure electricigenic bacteria strain Klebsiella oxytoca d7and Shewanella sp. F1 were constructed respectively, and the mechanisms of electron transfer by the electron transfer mediators (ETMs) during their electricity generation were studied. K. oxytoca d7 could only secrete ETMs during its electricigenic respiration, while the Shewanella sp. F1 could secrete ETMs during either its aerobiotic respiration, anaerobic respiration or electricigenic respiration, which indicated that the generation of ETMs was related to the species of electricigens. The redox potentials of the two species of bacteria were equivalent (-250~210mV), which were between that of the NADH and coenzyme Q in respiratory chain. The electrons intercepted by the ETMs were both denoted from the NADH on the intracellular respiratory chain, indicating that the "escape" site of the electron only depended on the ETMs itself. Under the conditions of sufficient or insufficient carbon sources, in terms of the K. oxytoca d7, the amount of electricity produced by the ETMs accounted for 60% and 41% of the total electricity generated, respectively, while that of the Shewanella sp. F1 was 57% and 50%, respectively. Electricigenic respiration of two electricigens was mainly based on the ETMs mechanism. The conversion of anode substrate and the power generation performance of the MFCs was directly affected by the process of electron transfer mediated by the ETMs.
Keywords:electricigenic respiration  electron transfer mediator  electricigens  microbial fuel cell  
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