基于细胞尺度的光照对四尾栅藻生长的影响

以四尾栅藻（Scenedesmus quadricauda）为研究对象，采用平行平板流动腔装置，基于计算机视觉的藻细胞动态生长观察方法，从单细胞尺度研究不同光照强度对四尾栅藻藻细胞生长的影响.成功建立了四尾栅藻个体生长曲线模型，模型拟合效果良好.结果表明：在8000lux光照强度下，藻细胞的体积最大比生长速率最大，即四尾栅藻生长的最适光照强度为8000lux；适宜的光照条件可以增加藻细胞分裂时的大小，小于8000lux时，藻细胞分裂体积随着光照强度的增加而增加，大于8000lux时，藻细胞分裂时体积反而越来越小；较高的光照强度还有利于藻细胞适应新的环境，减少藻细胞复苏时间.

Effect of light intensity on the growth of Scenedesmus quadricauda at cell scale

Achievements have been made on the research in the influence of light on the algal growth. While the mechanism of the influence remains unclear in many aspects. The dynamic growth of Scenedesmus quadricauda cells was observed with the parallel plate flow chamber device and the computer vision technology in the study. The effect of different light intensity on the growth of Scenedesmus quadricauda were studied at a single cell scale. The individual growth curve model of Scenedesmus quadricauda has been established, which fitted the measured data well. The results showed that the largest maximum volumic growth rate of algal cells appeared under 8000lux light intensity, which could be regarded as the optimal light intensity for Scenedesmus quadricauda. The appropriate light conditions could increase algal cell's size before division. The algal cell's size before division increased with the increase of light intensity when it was lower than 8000lux. While the size decreased with the increase of light intensity when it was higher than 8000lux. In addition, higher light intensity was advantageous for an algal cell to adapt to the new environment and to reduce the time required for the algae recruitment.