好氧颗粒污泥及其高效菌株降解苯胺的试验研究

以苯胺为唯一碳源和氮源,培养降解高浓度苯胺废水的好氧颗粒污泥,该体系对苯胺废水的最高耐受浓度高达6 000mg/L.通过分离纯化,从颗粒污泥体系中获得2株具有不同降解特征的苯胺降解菌adx1和adx3,菌株adx1在降解速率上具有明显优势,而菌株adx3对苯胺的最高耐受浓度高于adx1.上述菌株在降解苯胺过程中均遵循Haldane动力学模型,菌株adx1和adx3的最大比较降解速率分别为0.924 g/(g.h)和0.645 g/(g.h),比生长速率分别为0.487 g/(g.h)和0.440 g/(g.h).16S rDNA测序结果表明adx1和adx3分别属于Pseudomonas和Achromobacter属,与好氧颗粒污泥PCR-DGGE指纹图条带1和4测序结果一致,表明上述菌株分别为好氧颗粒化体系中优势菌群之一.

Aniline Removal by Aerobic Granules and High-Efficiency Aniline-Degrading Bacteria

Aerobic granules were successfully cultivated with aniline as the sole source of carbon and nitrogen. This system could effectively degrade aniline as high as 6 000 mg/L. Two aniline-degrading strains which were named as adxl and adx3, were isolated and purified from the aerobic granular sludge. The degradation characteristics of these two bacteria were different. The strain adxl possessed a relatively higher degradation rate of aniline, while the strain adx3 could stand up to a higher concentration of aniline than the strain adx1. The degrading process of these two strains followed the Haldane kinetic model. The maximum aniline biodegradation rates of adxl and adx3 were up to 0.924 g/(g·h)and 0.645 g/(g·h), respectively. The maximum specific growth rates were as high as 0.487 g/(g·h) and 0.440 g/(g·h), respectively.Identification by 16S rDNA gene sequence analysis showed that adxl and adx3 were Pseudomonas sp. and Achromobacter sp. , which was consistent with the identification by the bands 1 and 4 in the PCR-DGGE profile of the aerobic aniline-degrading granules. Therefore, it could be proposed that die strains adxl and adx3 were one of the main functional microorganisms inhabited in the aerobic granules.