五氯酚和八氯代二苯并二噁英复合暴露对斑马鱼胚胎发育的毒性效应

为了评价环境中五氯酚(PCP)和八氯代二苯并二噁英(OCDD)对水环境以及鱼类的影响,以斑马鱼为模式生物,研究了PCP和OCDD对其胚胎发育的单一及复合毒性效应.结果表明,PCP单独暴露(浓度25μg·L-1～5mg·L-1)对斑马鱼胚胎发育具有较强的毒性效应,可导致胚胎孵化率显著下降,死亡率、畸形率显著上升,而OCDD单独暴露(200、500μg·L-1)对斑马鱼胚胎发育没有明显的毒性效应;OCDD与环境浓度的PCP复合暴露(OCDD+PCP1:250μg·L-1+25μg·L-1;OCDD+PCP2:250μg·L-1+50μg·L-1)对斑马鱼胚胎的存活与发育等没有显著影响,对斑马鱼胚胎内CYP1A基因表达以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的酶活力也没有显著影响,在实验浓度下二者共存没有明显的复合毒性效应。

Combined Toxicity of Pentachlorophenol(PCP)and Octachlorodibenzo-p-dioxin(OCDD)on Development of Zebrafish(Danio rerio)Embryos

In order to assess the effect of pentachlorophenol(PCP)and octachlorodibenzo-p-dioxin(OCDD)on aquatic environment and fish, we studied the single and combined toxicity of PCP and OCDD using zebrafish as the model animal. The results showed that PCP(25μg·L-1～5mg·L-1)could cause severe developmental toxicity, while no effects were observed by OCDD(200μg·L-1 and 500μg·L-1). Embryos exposed to PCP showed a significant reduction in hatching rate while increase in mortality and malformation rate. No obvious effect on survival and development was found for the combined exposure of PCP and OCDD(OCDD+PCP1: 250μg·L-1+25μg·L-1; OCDD+PCP2: 250μg·L-1+50μg·L-1), and there were no significant differences in CYP1A expression and activities of SOD and CAT between the co-exposed embryos and the control. In conclusion, our results showed that there was no obvious combined toxicity to zebrafish embryos at the experimental exposure concentrations.