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微囊藻毒素导致鲫鱼淋巴细胞凋亡的研究
引用本文:张建英,张杭君,陈英旭.微囊藻毒素导致鲫鱼淋巴细胞凋亡的研究[J].环境科学学报,2005,25(8):1101-1104.
作者姓名:张建英  张杭君  陈英旭
作者单位:浙江大学环境与资源学院 杭州310028 (张建英,张杭君),浙江大学环境与资源学院 杭州310028(陈英旭)
基金项目:浙江省科学技术厅资助项目(N0.2003C33040)
摘    要:采用离体细胞培养诱导方法,以鲫鱼(Garassiusauratus)为实验材料,研究了两种微囊藻毒素microcystinLR(MCLR)和microcystinRR(MCRR)在低浓度下(1nmol·L-1,5nmol·L-1和10nmol·L-1)对鲫鱼淋巴细胞的毒性效应.结果表明,鲫鱼淋巴细胞分别经两种微囊藻毒素体外诱导2h后,出现细胞核固缩的典型细胞凋亡形态学特征;琼脂糖凝胶电泳检测到明显的细胞凋亡的生物化学特征梯状DNA(DNAladder);并且两种藻毒素诱导的细胞凋亡呈时间和剂量效应关系.该研究表明微囊藻毒素可导致鱼类淋巴细胞产生凋亡,因而可能影响鱼类免疫功能.

关 键 词:微囊藻毒素  细胞凋亡  淋巴细胞
文章编号:0253-2468(2005)08-1101-04
收稿时间:02 17 2005 12:00AM
修稿时间:06 6 2005 12:00AM

Induction of apoptosis in the lymphocytes of the crucian carp (Garassius auratus) by microcystins
ZHANG Jianying,ZHANG Hangjun and CHEN Yingxu.Induction of apoptosis in the lymphocytes of the crucian carp (Garassius auratus) by microcystins[J].Acta Scientiae Circumstantiae,2005,25(8):1101-1104.
Authors:ZHANG Jianying  ZHANG Hangjun and CHEN Yingxu
Institution:College of Environmental Science and Resources, Zhejiang University, Hangzhou 310028,College of Environmental Science and Resources, Zhejiang University, Hangzhou 310028 and College of Environmental Science and Resources, Zhejiang University, Hangzhou 310028
Abstract:The mercury exchange fluxes between soil and air in Chongqing were measured using RA-915+ mercury analyzer and dynamic flux chamber technique. The surface types of ground, including forestland, grassland and cultivated land were studied. The results indicated that the mercury exchange fluxes were from -59 to 733.8ng·m-2·h-1, and the average was 115ng·m-2·h-1. The mercury exchange flux was in the order of polluted district (weather station) > cultivated land > grassland > forestland. There were close relationships between mercury fluxes and environmental parameters, such as mercury content of soil, solar radiation, relative humidity, soil temperature, and so on. This indicated that environmental factors had a strong impact on mercury emission fluxes from soil. Meanwhile, regional mercury emission was preliminary estimated based on the climate characteristic of Chongqing. The result showed that the total mercury amount released from forestland, cultivated land (upland) and grassland in Chongqing was 791kg in 2003, the emission intensity was 17.1g·km-2·a-1.
Keywords:microcystins  apoptosis  lymphocytes
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