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嗜麦芽寡养单胞菌DHHJ固态发酵羽毛工艺的研究
引用本文:梁田,吴小倩,曹张军,周美华. 嗜麦芽寡养单胞菌DHHJ固态发酵羽毛工艺的研究[J]. 环境工程学报, 2012, 6(1): 322-326
作者姓名:梁田  吴小倩  曹张军  周美华
作者单位:1. 东华大学环境科学与工程学院,上海,201620
2. 东华大学化学化工与生物工程学院,上海,201620
基金项目:上海市重点学科建设资助项目(B604);高等学校博士点基金资助项目(20090075110007)
摘    要:为了将微生物处理羽毛工艺产业化,利用课题组筛选得到的嗜麦芽寡养单胞菌,在液态发酵羽毛工艺的基础上进行固态发酵工艺的研究。通过发酵条件和培养基成分的优化,确定了嗜麦芽寡养单胞菌固态发酵羽毛的工艺为:初始pH为7.5,培养温度为35℃,培养时间为4 d,培养基组成是麸皮和干酪素,固液比是1∶1.5,照此工艺发酵,发酵液中蛋白质含量为23.78 mg/mL,角蛋白酶活为20.46 U/mL,菌株的产酶能力及降解羽毛能力显著高于液态发酵条件。

关 键 词:嗜麦芽寡养单胞菌  固态发酵  羽毛

Study on solid-state fermentation of feather with Stenotrophomonas maltophilia DHHJ
Liang Tian,Wu Xiaoqian,Cao Zhangjun and Zhou Meihua. Study on solid-state fermentation of feather with Stenotrophomonas maltophilia DHHJ[J]. Techniques and Equipment for Environmental Pollution Control, 2012, 6(1): 322-326
Authors:Liang Tian  Wu Xiaoqian  Cao Zhangjun  Zhou Meihua
Affiliation:1(1.College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China; 2.College of Chemistry,Chemical Engineering and Biological Engineering,Donghua University,Shanghai 201620,China)
Abstract:In order to industrialize the utilization of waste feather by microbiological method,the test of solid-state fermentation for feather with Stenotrophomonas maltophilia DHHJ was studied based on its liquid-state fermentation previously.The results showed that the optimal solid state fermentation was initial pH 7.5 at 35 ℃ for four days,the medium was composed of bran and casein,and the solid-liquid ratio was 1∶ 1.5.Under the optimal conditions,the abilities of keratinase production and feather degradation of strain DHHJ were much better than those under the liquid-state fermentation,the protein content was 23.78 mg/mL and the activity of keratinase was 20.46 U/mL.
Keywords:Stenotrophomonas maltophilia DHHJ  solid-state fermentation  feather
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