基于荧光素酶发光体系测试饮用水中农药的综合毒性

利用农药对荧光素酶催化的发光反应具有非常显著的抑制作用,对甲拌磷、乐果、毒死蜱、百草枯等4种农药分别进行单一毒性和等比混合法联合毒性测试,建立了一种快速检测饮用水中农药综合毒性的生物学方法。试验结果表明,单一农药乐果、甲拌磷、百草枯和毒死蜱的EC50值分别为7.56 mg/L、12.7 mg/L、19.0 mg/L和65.3 mg/L,毒性强弱顺序为乐果甲拌磷百草枯毒死蜱,相关系数≥0.995;将4种农药以等比方式配制成两两混合液后,当质量浓度为20.0 mg/L~100 mg/L时,除百草枯与毒死蜱表现为毒性协同外,其他两两混合农药的毒性以拮抗作用为主。

Determ ination of Synthetic Toxicity of Pesticidesin Drinking Water Based on Inhibition of Biolum inescence from Luciferase Reacting System

A fast determination method was established for synthetic toxicity in drinking water. Four pesticides （thimet, cygon, chlorpyrifos and paraquat） were used to investigate single toxicity and synthetic toxicity by classic method of equal dose-effect experiment with inhibition of bioluminescence from lueiferase reacting system. Results showed that EC50 were thimet 7.56 mg/L, eygon 12.7 mg/L, chlorpyrifos 19.0 mg/L and paraquat 65.3 mg/L respectively. Toxicity level order of the pesticides was following： cygon 〉 thimet 〉 paraquat 〉 chlorpyrifos. The related coefficient was ≥ 0. 995. When any two of the pesticides from range of 20.0 mg/L to 100 mg/L were mixed based on equal toxicity of single pesticide, antagonistic effect was manifested except that mixture of paraquat and ehlorpyrifos showed the synergetic effect.