A High Performance Liquid Chromatographic Determination of Free Amino Acids in Natural Waters in the Picomolar (M X 10-12) Range Suitable for Shipboard Use |
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| Authors: | Roger Evens James Braven Leslie Brown Ian Butler |
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| Institution: |
a Department of Environmental Sciences, Plymouth Polytechnic, Plymouth, Devon, U.K.
b Marine Biological Association of the U.K., Citadel Hill, Plymouth |
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| Abstract: | A reliable method for the determination of amino acids, suitable for routine shipboard use, is presented. Using reverse-phase high-performance liquid chromatography with computer-controlled gradient elution optimization, the amino acids commonly found in sea-water were clearly resolved and determined at the picomole level. A procedure was also developed to enable repeat injections of sea water samples to be made without deterioration of column material or performance. Thus the analysis was made more operator-independent and consequently more suitable for use at sea. |
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